Plasmodium falciparum associated with severe childhood malaria preferentially expresses PfEMP1 encoded by group A var genes
Autor: | Thomas Lavstsen, Anja T. R. Jensen, Lasse S Vestergaard, Lars Hviid, Pamela Magistrado, Trine Staalsoe, John Lusingu, Jesper Christensen, Rob Hermsen, Colin J. Sutherland, Ali Salanti, Morten Nielsen, Thor G. Theander, Antonella Chiucchiuini, Sarah Sharp, Robert W. Sauerwein, Louise Joergensen |
---|---|
Rok vydání: | 2004 |
Předmět: |
Transcription
Genetic var gene Immunology Genes Protozoan Molecular Sequence Data Plasmodium falciparum Clone (cell biology) Protozoan Proteins malaria Antibodies Protozoan Antigens Protozoan antibody selection Group A Polymerase Chain Reaction Article Antigen parasitic diseases medicine Antigenic variation Immunology and Allergy Animals Humans Amino Acid Sequence Cloning Molecular Malaria Falciparum Child Gene DNA Primers biology Base Sequence Sequence Homology Amino Acid Erythrocyte Membrane medicine.disease biology.organism_classification Phenotype Virology Recombinant Proteins PfEMP1 Gene Expression Regulation Microbial pathogenesis and host defense [UMCN 4.1] Sequence Alignment Malaria |
Zdroj: | Journal of Experimental Medicine, 199, 1179-90 Jensen, A T R, Magistrado, P, Sharp, S, Joergensen, L, Lavstsen, T, Chiucchiuini, A, Salanti, A, Vestergaard, L S, Lusingu, J P, Hermsen, R, Sauerwein, R, Christensen, J, Nielsen, M A, Hviid, L, Sutherland, C, Staalsoe, T & Theander, T G 2004, ' Plasmodium falciparum associated with severe childhood malaria preferentially expresses PfEMP1 encoded by group A var genes ', Journal of Experimental Medicine, vol. 199, no. 9, pp. 1179-90 . https://doi.org/10.1084/jem.20040274 The Journal of Experimental Medicine Journal of Experimental Medicine, 199, 9, pp. 1179-90 |
ISSN: | 0022-1007 |
DOI: | 10.1084/jem.20040274 |
Popis: | Item does not contain fulltext Parasite-encoded variant surface antigens (VSAs) like the var gene-encoded Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) family are responsible for antigenic variation and infected red blood cell (RBC) cytoadhesion in P. falciparum malaria. Parasites causing severe malaria in nonimmune patients tend to express a restricted subset of VSA (VSA(SM)) that differs from VSA associated with uncomplicated malaria and asymptomatic infection (VSA(UM)). We compared var gene transcription in unselected P. falciparum clone 3D7 expressing VSA(UM) to in vitro-selected sublines expressing VSA(SM) to identify PfEMP1 responsible for the VSA(SM) phenotype. Expression of VSA(SM) was accompanied by up-regulation of Group A var genes. The most prominently up-regulated Group A gene (PFD1235w/MAL7P1.1) was translated into a protein expressed on the infected RBC surface. The proteins encoded by Group A var genes, such as PFD1235w/MAL7P1.1, appear to be involved in the pathogenesis of severe disease and are thus attractive candidates for a vaccine against life-threatening P. falciparum malaria. |
Databáze: | OpenAIRE |
Externí odkaz: |