Fluoride reactivation-enabled sensitive quantification of tabun adducts on human serum albumin by GC–MS/MS via isotope dilution
| Autor: | Zhong-Fang Xing, Ji-Na Wu, Long Yan, Yang Yang, Bo Chen, Xiao-Sen Li, Ling Yuan, Chang-Cai Liu |
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| Rok vydání: | 2019 |
| Předmět: |
isotope dilution
Chromatography Gas Clinical Biochemistry Indicator Dilution Techniques Serum Albumin Human 010501 environmental sciences Isotope dilution HSA adducts 01 natural sciences Analytical Chemistry Serine Fluorides chemistry.chemical_compound Tandem Mass Spectrometry medicine Humans acetone precipitation General Pharmacology Toxicology and Pharmaceutics 0105 earth and related environmental sciences Cholinesterase Tabun Nerve agent Chromatography Molecular Structure biology fluoride reactivation 010401 analytical chemistry GC–MS/MS Active site General Medicine Human serum albumin Organophosphates 0104 chemical sciences Medical Laboratory Technology chemistry biology.protein tabun Fluoride Research Article medicine.drug |
| Zdroj: | Bioanalysis |
| ISSN: | 1757-6199 1757-6180 |
| DOI: | 10.4155/bio-2019-0161 |
| Popis: | Organophosphorus nerve agents inhibit the cholinesterase activity by phosphylation of the active site serine. The resulting phosphylated cholinesterase and adducts on human serum albumin (HSA) are appropriate biomarkers for nerve agents exposure. Several methods have been developed for the detection of nerve agents, including fluoride reactivation or alkaline cleavage. It was previously thought that some nerve agents adducts to HSA could not be detected via fluoride regeneration. In our study, the results showed that tabun (GA) adducts of HSA could be detected by fluoride regeneration. The sample preparation included acetone precipitation, washing and SPE. Deuterated tabun (d5-GA) was applied as the internal standard. The product of regenerated fluorotabun is detected with a good linearity (R2 > 0.997) in the concentration range from 0.02 to 100.0 ng/ml, small relative standard deviation (≤6.89%) and favorable recoveries between 94.8 and 106.3%. The established preparation confirmed the fluorotabun was regenerated from the GA-HSA adducts. Graphical abstract Flow diagram for detection and quantitation of tabun adducts to human serum albumin via fluoride reactivation by GC–MS/MS using isotope dilution. FGA: Fluorotabun; GA: Tabun; HSA: Human serum albumin. |
| Databáze: | OpenAIRE |
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