Phenylpropanoids in Silybum marianum cultures treated with cyclodextrins coated with magnetic nanoparticles
| Autor: | Purificación Corchete, Lorena Almagro, Jose Antonio Gabaldón, María Angeles Pedreño, Javier Palazón |
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| Rok vydání: | 2022 |
| Předmět: |
Flavonoids
Cyclodextrins 2407.01 Cultivo Celular Polymers General Medicine t-resveratrol Methyl jasmonate Applied Microbiology and Biotechnology Antioxidants Hydroxypropyl-β-cyclodextrin polymer-coated magnetic nanoparticles 2417 Biología Vegetal (Botánica) Resveratrol Naringenin Silybum marianum suspension cultures Milk Thistle Magnetite Nanoparticles Biotechnology |
| Zdroj: | GREDOS. Repositorio Institucional de la Universidad de Salamanca instname |
| Popis: | [EN] The glucose oligosaccharide-derived cyclodextrins (CDs) are used for improving bioactive compound production in plant cell cultures because, in addition to their elicitation activity, CDs promote product removal from cells. However, despite these advantages, the industrial application of CDs is hampered by their high market price. A strategy to overcome this constraint was recently tested, in which reusable CD polymers coated with magnetic Fe3O4 nanoparticles were harnessed in Vitis vinifera cell cultures to produce t-resveratrol (t-R). In this study, we applied hydroxypropyl-β-CDs (HPCD) and HPCDs coated with magnetic nanoparticles (HPCD-EPI-MN) in methyl jasmonate (MJ)-treated transgenic Silybum marianum cultures ectopically expressing either a stilbene synthase gene (STS) or a chalcone synthase gene (CHS), and compared their efects on the yields of t-R and naringenin (Ng), respectively. HPCD-EPI-MN at 15 g/L stimulated the accumulation of metabolites in the culture medium of the corresponding transgenic cell lines, with up to 4 mg/L of t-R and 3 mg/L of Ng released after 3 days. Similar amounts were produced in cultures treated with HPCD. Concentrations higher than 15 g/L of HPCD-EPI-MN and prolonged incubation periods negatively afected cell growth and viability in both transgenic cell lines. Reutilization of HPCD-EPI-MN was possible in three elicitation cycles (72 h each), after which the polymer retained 25–30% of its initial efciency, indicating good stability and reusability. Due to their capacity to adsorb metabolites and their recyclability, the application of magnetic CD polymers may reduce the costs of establishing efcient secondary metabolite production systems on a commercial scale. Publicación en abierto financiada por el Consorcio de Bibliotecas Universitarias de Castilla y León (BUCLE), con cargo al Programa Operativo 2014ES16RFOP009 FEDER 2014-2020 DE CASTILLA Y LEÓN, Actuación:20007-CL - Apoyo Consorcio BUCLE. |
| Databáze: | OpenAIRE |
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