A genotype VII Newcastle disease virus-like particles confer full protection with reduced virus load and decreased virus shedding
| Autor: | Yongshan Wang, Wei Ouyang, Jindou Li, Jing Qian, Qianliang Yuan, Jiaxin Ding, Zhuang Ding, Yanlong Cong, Renfu Yin, Weiqi Wang, Xiaohong Xu |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
animal structures
Genotype Myeloma protein Newcastle Disease animal diseases viruses 030231 tropical medicine Newcastle disease virus Virulence Biology Antibodies Viral Vaccines Attenuated Newcastle disease Virus 03 medical and health sciences 0302 clinical medicine Animals Vaccines Virus-Like Particle 030212 general & internal medicine Viral shedding Poultry Diseases General Veterinary General Immunology and Microbiology Vaccination Public Health Environmental and Occupational Health Viral Vaccines Viral Load biochemical phenomena metabolism and nutrition biology.organism_classification Virology Virus Shedding Infectious Diseases Infectious disease (medical specialty) embryonic structures Molecular Medicine Chickens Viral Fusion Proteins |
| Zdroj: | Vaccine. 37:444-451 |
| ISSN: | 0264-410X |
| DOI: | 10.1016/j.vaccine.2018.11.068 |
| Popis: | Newcastle disease (ND) is one of the most severe avian infectious disease inflicting a great loss on poultry industry worldwide. The control of ND relies on proper vaccination strategies. The vaccine strains of Newcastle disease virus (NDV) mainly belong to genotype I, II or III, which cannot fully prohibit virus shedding against the prevalent genotype VII virulent strain attack. To develop a safe, genotype matched vaccine candidate, we employed a bac-to-bac expression system and constructed a genotype VII NDV strain based virus-like particles (NDV VLPs). It was constructed with NDV M protein as the skeleton, and protective antigen F and HN proteins displayed on the surface. The NDV VLPs exhibited a similar appearance to the live NDV particles, but with denser F and HN proteins displayed on the surface. The immunization assay indicated that NDV VLPs stimulated a longer protection period, less tissue virus loading and shorter virus shedding period than the commercialized LaSota-formulated vaccine when challenged with genotype VII NDV strain. These results proposed the potential role of NDV VLPs as an alternative to current live genotype unmatched vaccine for the control and eliminate NDV in the avian flocks. |
| Databáze: | OpenAIRE |
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