Microsatellites cross-species amplification across some african cichlids
| Autor: | Etienne Bezault, Jean-François Baroiller, Bernard Chevassus, Xavier Rognon, Karim Gharbi |
|---|---|
| Přispěvatelé: | Intensification raisonnée et écologique pour une pisciculture durable (UMR INTREPID), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Génétique Animale et Biologie Intégrative (GABI), AgroParisTech-Institut National de la Recherche Agronomique (INRA), Department of Biology, Reed College, Institute of Evolutionary Biology, School of Biological Sciences, University of Edinburgh, This study was supported by grants from the CIRAD and INRA (France)., Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad) |
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
0106 biological sciences
food.ingredient Article Subject Polymorphisme génétique [SDV]Life Sciences [q-bio] satellite Zoology Locus (genetics) Aquaculture amplification 010603 evolutionary biology 01 natural sciences 03 medical and health sciences Nile tilapia food Sensu Cichlid Sarotherodon cichlid Marqueur génétique 14. Life underwater Tilapia (genre) 030304 developmental biology Génie génétique 0303 health sciences biology Ecology Oreochromis niloticus Microsatellite Tilapia Cichlidae L10 - Génétique et amélioration des animaux biology.organism_classification Oreochromis M12 - Production de l'aquaculture human activities Research Article |
| Zdroj: | International Journal of Evolutionary Biology International Journal of Evolutionary Biology, 2012, 2012, Non paginé. ⟨10.1155/2012/870935⟩ International Journal of Evolutionary Biology (2012), Non paginé. (2012) Bezault, E, Rognon, X, Gharbi, K, Baroiller, J-F & Chevassus, B 2012, ' Microsatellites Cross-Species Amplification across Some African Cichlids ' vol. 2012, 870935 . DOI: 10.1155/2012/870935 |
| ISSN: | 2090-052X |
| DOI: | 10.1155/2012/870935⟩ |
| Popis: | The transfer of the genomic resources developed in the Nile tilapia,Oreochromis niloticus, to other Tilapiinessensu latoand African cichlid would provide new possibilities to study this amazing group from genetics, ecology, evolution, aquaculture, and conservation point of view. We tested the cross-species amplification of 32O. niloticusmicrosatellite markers in a panel of 15 species from 5 different African cichlid tribes: Oreochromines (Oreochromis, Sarotherodon), Boreotilapiines (Tilapia), Chromidotilapines, Hemichromines, and Haplochromines. Amplification was successfully observed for 29 markers (91%), with a frequency of polymorphic (P95) loci per species around 70%. The mean number of alleles per locus and species was 3.2 but varied from 3.7 withinOreochromisspecies to 1.6 within the nontilapia species. The high level of cross-species amplification and polymorphism of the microsatellite markers tested in this study provides powerful tools for a wide range of molecular genetic studies within tilapia species as well as for other African cichlids. |
| Databáze: | OpenAIRE |
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