Characterization and activity of phospholipase A2 in normal human epidermis and in lesion-free epidermis of patients with psoriasis or eczema
| Autor: | H.J. Yardley, R. Summerly, Elizabeth Ilderton, J. F. B. Norris, Susan Forster |
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| Rok vydání: | 1985 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Time Factors Adolescent Eczema Dermatology Biology Phospholipases A Pathogenesis Lesion Phospholipase A2 Dermis Psoriasis Biopsy medicine Humans Aged chemistry.chemical_classification Epidermis (botany) medicine.diagnostic_test Hydrolysis Proteins Hydrogen-Ion Concentration Middle Aged medicine.disease Molecular biology Phospholipases A2 medicine.anatomical_structure Enzyme chemistry Phospholipases Phosphatidylcholines biology.protein Calcium Female Epidermis medicine.symptom |
| Zdroj: | British Journal of Dermatology. 112:135-147 |
| ISSN: | 1365-2133 0007-0963 |
| DOI: | 10.1111/j.1365-2133.1985.tb00077.x |
| Popis: | SUMMARY The kinetic properties of phospholipase A2 isolated from single large specimens of normal human epidermis and‘uninvolved’(lesion-free) psoriatic epidermis were determined. The enzymes from the two sources behaved identically with respect to changes in protein concentration, Ca2+ concentration and pH, but the enzymes responded differently to changes in substrate concentration. Furthermore, the specific activity of the enzyme derived from lesion-free psoriatic epidermis was higher than that from normal epidermis under all conditions used. Increased specific activity of the enzyme in the lesion-free epidermis was also found when biopsy specimens taken from thirty-five patients with psoriasis vulgaris at varying severity were compared with biopsies of normal epidermis from thirty-one control volunteers (P < 0.001). Mixing experiments, in which homogenates of lesion-free psoriatic epidermis and control epidermis were combined, suggested that the relatively low activity of the enzyme in normal epidermis was due to the presence of an inhibitor. As the activity of the enzyme was not elevated in the lesion-free epidermis from twelve cases of eczema, which is also an inflammatory condition of the epidermis and superficial dermis, it is suggested that the raised phospholipase A2 activity demonstrated in the lesion-free epidermis of psoriasis may play an important role in the pathogenesis of the disease. |
| Databáze: | OpenAIRE |
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