Microbiota, metabolome, and immune alterations in obese mice fed a high-fat diet containing type 2 resistant starch
| Autor: | Alice Martinic, Darya O. Mishchuk, Yu-Hsin Hsieh, Roy J. Martin, Jose Zaragoza, Javad Barouei, Dorothy A. Kieffer, Carolyn M. Slupsky, Maria L. Marco, Zach Bendiks, Dustin D. Heeney |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty food.ingredient Firmicutes Diet High-Fat 03 medical and health sciences chemistry.chemical_compound Immune system food Ileum Internal medicine Metabolome medicine Animals Metabolomics Obesity Intestinal Mucosa Resistant starch Cecum Immunity Mucosal Bifidobacterium Principal Component Analysis biology Bacteroidetes Lactobacillales Gene Expression Profiling Resistant Starch Starch Maltose biology.organism_classification medicine.disease Mice Inbred C57BL 030104 developmental biology Endocrinology Gene Expression Regulation Liver chemistry Dysbiosis Digestion Adiponectin Diet Carbohydrate Loading medicine.symptom Weight gain Biomarkers Food Science Biotechnology |
| Zdroj: | Molecular Nutrition & Food Research. 61:1700184 |
| ISSN: | 1613-4125 |
| Popis: | cope : We examined the intestinal and systemic responses to incorporating a type 2 resistant starch (RS) into a high fat diet fed to obese mice. Methods and results : Diet-induced obese, C57BL/6J mice were fed a HF diet without or with 20% (by weight) high-amylose maize resistant starch (HF-RS) for six weeks. Serum adiponectin levels were higher with RS consumption, but there were no differences in weight gain and adiposity. With HF-RS, the expression levels of ileal TLR2 and Reg3g and cecal occludin, TLR2, TLR4, NOD1 and NOD2 were induced; whereas colonic concentrations of the inflammatory cytokine IL-17A declined. The intestinal, serum, liver, and urinary metabolomes were also altered. HF-RS resulted in lower amino acid concentrations, including lower serum branched chain amino acids, and increased quantities of urinary di/trimethylamine, 3-indoxylsulfate, and phenylacetylglycine. Corresponding to these changes were enrichments in Bacteroidetes (S24-7 family) and certain Firmicutes taxa (Lactobacillales and Erysipelotrichaceae) with the HF-RS diet. Parabacteroides and S24-7 positively associated with cecal maltose concentrations. These taxa and Erysipelotrichaceae, Allobaculum, and Bifidobacterium were directly correlated with uremic metabolites. Conclusion : Consumption of RS modified the intestinal microbiota, stimulated intestinal immunity and endocrine-responses, and modified systemic metabolomes in obese mice consuming an otherwise obesogenic diet. This article is protected by copyright. All rights reserved |
| Databáze: | OpenAIRE |
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