The ovine hepatic mitochondrial proteome: Understanding seasonal weight loss tolerance in two distinct breeds
| Autor: | Johan Greeff, Tim Scanlon, Tanya Kilminster, Alexandre Campos, Aspinas Chapwanya, John Milton, André M. Almeida, C. M. Oldham, Blake A. Miller, Don R. Bergfelt, Hugo Osório |
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| Přispěvatelé: | Instituto de Investigação e Inovação em Saúde |
| Rok vydání: | 2019 |
| Předmět: |
Proteome
Protein metabolism Mitochondria Liver Breeding Pathology and Laboratory Medicine Biochemistry Polyacrylamide Gel Electrophoresis chemistry.chemical_compound hepatic mitochondrial proteome Weight loss Medicine and Health Sciences Blue Native Polyacrylamide Gel Electrophoresis Immune Response Damara sheep Energy-Producing Organelles Gel Electrophoresis Protein Metabolism Mammals 2. Zero hunger 0303 health sciences Multidisciplinary biology Fatty Acids Eukaryota Neurochemistry Neurotransmitters Ruminants 04 agricultural and veterinary sciences Lipids Breed Mitochondria 3. Good health Vertebrates Medicine Seasons Cellular Structures and Organelles Glutamate medicine.symptom Research Article breed Science Immunology biology.animal_breed Bioenergetics Research and Analysis Methods Mitochondrial Proteins Andrology Electrophoretic Techniques 03 medical and health sciences Signs and Symptoms Diagnostic Medicine Weight Loss medicine Animals Nutrition 030304 developmental biology Inflammation Sheep Fatty acid metabolism Glutamate dehydrogenase Organisms 0402 animal and dairy science Biology and Life Sciences Cell Biology Metabolism 040201 dairy & animal science Diet ovine chemistry Amniotes weight loss Neuroscience |
| Zdroj: | PLoS ONE, Vol 14, Iss 2, p e0212580 (2019) Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP PLoS ONE |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0212580 |
| Popis: | Seasonal weight loss (SWL) is a primary constraint for farmers in the Mediterranean and tropics. One cost-effective solution to SWL is utilizing breeds like the Damara sheep that have adapted to deal with nutritional stress. Previous studies concluded that one of the adaptation mechanisms of SWL is a specialized fatty acid metabolism. Accordingly, hepatic-mitochondrial proteomes were compared across two different breeds (24 sheep total, Merino, n = 12 and Damara, n = 12) and two different diets (restricted vs unrestricted diet, 6 per breed, per diet, 24 total). Mitochondrial-proteins were isolated and relatively quantified using Blue native PAGE / 2D-electrophoresis and then analyzed via mass spectrometry. The tool ReviGO summarized the proteomes’ gene-ontology terms. A total of 50 proteins were identified with 7 changing significantly in abundance (ANOVA p-value |
| Databáze: | OpenAIRE |
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