Anti-human immunodeficiency virus type 1 antibodies of noninfected subjects are not related to autoantibodies occurring in systemic diseases
| Autor: | R Kämmerer, P Bürgisser, P C Frei |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Male
Microbiology (medical) viruses Blotting Western Clinical Biochemistry Immunology HIV Core Protein p24 Disease Cross Reactions HIV Antibodies Autoantigens snRNP Core Proteins Epitope Autoimmune Diseases Mixed connective tissue disease Western blot Predictive Value of Tests immune system diseases HIV Seropositivity medicine Humans Immunology and Allergy Aged Autoantibodies biology medicine.diagnostic_test SnRNP Core Proteins Autoantibody virus diseases Ribonucleoproteins Small Nuclear medicine.disease Virology HIV-1 biology.protein Antibody Indeterminate Research Article |
| Zdroj: | Clinical Diagnostic Laboratory Immunology. 2:458-461 |
| ISSN: | 1098-6588 1071-412X |
| DOI: | 10.1128/cdli.2.4.458-461.1995 |
| Popis: | Indeterminate Western blot (WB) (immunoblot) patterns for anti-human immunodeficiency virus type 1 (HIV-1) antibodies are often observed when testing serum samples from noninfected individuals. We investigated here the possible involvement of some frequently occurring autoantibodies (anti-SmB/B', U1snRNP [68 kDa, A, and C], Ro/SS-A [60 and 52 kDa], and Jo-1) in the generation of such indeterminate HIV-1 WB. In particular, the role of a reported sequence homology between p24 gag and the SmB/B' autoantigen was investigated. Serum samples were obtained from 50 healthy controls, 51 patients with systemic lupus erythematosus (SLE), 46 with systemic sclerosis, 6 with Sjögren's disease, 3 with mixed connective tissue disease, and 41 healthy subjects with persistent indeterminate HIV-1 WB. Reactivity to HIV-1 p24 gag was slightly but not significantly more frequent in patients with SLE than in controls (25.5% versus 14.0%; P > 0.1), whereas reactivity to HIV-1 p17 gag was significantly more frequent in the former subjects (23.5% versus 8.0%; P = 0.03). Simultaneous reactivity to p17 and p24 was observed in patients with SLE (11.8%; P = 0.014) or systemic sclerosis (8.7%; P = 0.049) but not in controls. There was no association found between the presence of any autoantibody and the occurrence of indeterminate HIV-1 WB nor between the presence of p24-reactive antibodies and anti-SmB/B'; this indicates that most p24-reactive antibodies are directed to epitopes other than the proline-rich sequences shared by p24 gag and SmB/B'. |
| Databáze: | OpenAIRE |
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