DNA methylation in the OPG/RANK/RANKL pathway is associated with steroid-induced osteonecrosis of the femoral head
| Autor: | Xiaolong Yang, Menghu Sun, Huiqiang Wu, Yuju Cao, Jianzhong Wang, Feimeng An |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
musculoskeletal diseases
Male medicine.medical_specialty Diseases of the musculoskeletal system RANK Methylation 03 medical and health sciences Femoral head 0302 clinical medicine Rheumatology Femur Head Necrosis Internal medicine medicine Humans Orthopedics and Sports Medicine Gene 030304 developmental biology 0303 health sciences biology business.industry RANK Ligand RANKL Osteoprotegerin Femur Head DNA Methylation Hedgehog signaling pathway medicine.anatomical_structure RC925-935 CpG site 030220 oncology & carcinogenesis Case-Control Studies DNA methylation biology.protein Cancer research OPG Female Steroids business Steroid-induced ONFH Research Article |
| Zdroj: | BMC Musculoskeletal Disorders BMC Musculoskeletal Disorders, Vol 22, Iss 1, Pp 1-12 (2021) |
| ISSN: | 1471-2474 |
| Popis: | Background Dysregulation of the OPG/RANK/RANKL signalling pathway is a key step in the occurrence of steroid-induced osteonecrosis of the femoral head (ONFH). This study aims to understand the degree of methylation of the OPG, RANK, and RANKL genes in steroid-related ONFH. Methods A case-control study was designed, including 50 patients (25 males and 25 females) and 50 matched controls. The European Molecular Biology Open Software Suite (EMBOSS) was used to predict the existence and location of CpG islands in the OPG, RANK, and RANKL genes. The Agena MassARRAY platform was used to detect the methylation status of the above genes in the blood of subjects. The relationship between the methylation level of CpG sites in each gene and steroid-related ONFH was analysed by the chi-square test, logistic regression analysis, and other statistical methods. Results In the CpG islands of the OPG, RANK, and RANKL genes in patients with steroid-related ONFH, several CpG sites with high methylation rates and high methylation levels were found. Some hypermethylated CpG sites increase the risk of steroid-related ONFH. In addition, a few hypermethylated CpG sites have predictive value for the early diagnosis of steroid-related ONFH. Conclusion Methylation of certain sites in the OPG/RANK/RANKL signalling pathway increases the risk of steroid-related ONFH. Some hypermethylated CpG sites may be used as early prediction and diagnostic targets for steroid-related ONFH. |
| Databáze: | OpenAIRE |
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