Circadian rhythms of locomotor activity and hippocampal clock genes expression are dampened in vitamin A–deficient rats
| Autor: | Ana Cecilia Anzulovich, Rebeca S. Golini, Lorena S. Navigatore-Fonzo, Silvia Marcela Delgado |
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| Rok vydání: | 2014 |
| Předmět: |
Male
medicine.medical_specialty ACTIVIY/REST CYCLE Light CIRCADIAN RHYTHM CRY Biología Photoperiod Endocrinology Diabetes and Metabolism Period (gene) Circadian clock Gene Expression Endogeny Motor Activity Biology Hippocampus Ciencias Biológicas Rats Sprague-Dawley ENDOGENOUS CLOCK LOCOMOTOR ACTIVITY Endocrinology Cryptochrome Biological Clocks Internal medicine medicine Animals Circadian rhythm Vitamin A Nutrition and Dietetics Vitamin A Deficiency Suprachiasmatic nucleus Period Circadian Proteins Glutathione Circadian Rhythm Rats PER CLOCK PER2 Gene Expression Regulation HIPPOCAMPUS VITAMIN A-DEFICIENT RATS Oxidation-Reduction BIOLOGICAL RHYTHM CIENCIAS NATURALES Y EXACTAS |
| Zdroj: | Nutrition Research. 34:326-335 |
| ISSN: | 0271-5317 |
| DOI: | 10.1016/j.nutres.2014.02.002 |
| Popis: | The main external time giver is the day-night cycle; however, signals from feeding and the activity/rest cycles can entrain peripheral clocks, such as the hippocampus, in the absence of light. Knowing that vitamin A and its derivatives, the retinoids, may act as regulators of the endogenous clock activity, we hypothesized that the nutritional deficiency of vitamin A may influence the locomotor activity rhythm as well as the endogenous circadian patterns of clock genes in the rat hippocampus. Locomotor activity was recorded during the last week of the treatment period. Circadian rhythms of clock genes expression were analyzed by reverse transcription–polymerase chain reaction in hippocampus samples that were isolated every 4 hours during a 24-hour period. Reduced glutathione (GSH) levels were also determined by a kinetic assay. Regulatory regions of clock PER2, CRY1, and CRY2 genes were scanned for RXRE, RARE, and RORE sites. As expected, the locomotor activity pattern of rats shifted rightward under constant dark conditions. Clock genes expression and GSH levels displayed robust circadian oscillations in the rat hippocampus. We found RXRE and RORE sites on regulatory regions of clock genes. Vitamin A deficiency dampened rhythms of locomotor activity as well as modified endogenous rhythms of clock genes expression and GSH levels. Thus, vitamin A may have a role in endogenous clock functioning and participate in the circadian regulation of the cellular redox state in the hippocampus, a peripheral clock with relevant function in memory and learning. Fil: Navigatore Fonzo, Lorena Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis; Argentina Fil: Delgado, Silvia M.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina Fil: Golini, Rebeca Laura Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis; Argentina Fil: Anzulovich Miranda, Ana Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis; Argentina |
| Databáze: | OpenAIRE |
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