In vitro antiplasmodial activity of Withania frutescens —Solanaceae
| Autor: | Mohamed Markouk, Evelyne Ollivier, M. Larhsini, Widad Ben Bakrim, Khalid Bekkouche, Nadine Azas, Valérie Mahiou, Laila El Bouzidi |
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| Přispěvatelé: | Institut méditerranéen de biodiversité et d'écologie marine et continentale (IMBE), Centre National de la Recherche Scientifique (CNRS)-Institut de recherche pour le développement [IRD] : UMR237-Aix Marseille Université (AMU)-Avignon Université (AU), Laboratory of Pharmacognosy and Ethnopharmacology, Infections Parasitaires : Transmission, Physiopathologie et Thérapeutiques (IP-TPT), Service de Santé des Armées-Assistance Publique - Hôpitaux de Marseille (APHM)-Aix Marseille Université (AMU)-Institut de Recherche pour le Développement (IRD), Avignon Université (AU)-Aix Marseille Université (AMU)-Institut de recherche pour le développement [IRD] : UMR237-Centre National de la Recherche Scientifique (CNRS), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Assistance Publique - Hôpitaux de Marseille (APHM)-Service de Santé des Armées |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Antiparasitic
medicine.drug_class Cytotoxicity [SDV]Life Sciences [q-bio] Flavonoid Plasmodium falciparum Ethyl acetate 01 natural sciences chemistry.chemical_compound medicine [SDV.BV]Life Sciences [q-bio]/Vegetal Biology chemistry.chemical_classification biology Traditional medicine 010405 organic chemistry Butanol biology.organism_classification Withania frutescens 3. Good health 0104 chemical sciences [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy 010404 medicinal & biomolecular chemistry Complementary and alternative medicine Phytochemical chemistry Polyphenol Antiplasmodial activity Selectivity index Solanaceae |
| Zdroj: | European Journal of Integrative Medicine European Journal of Integrative Medicine, Elsevier, 2017, 14, pp.28-31. ⟨10.1016/j.eujim.2017.08.009⟩ European Journal of Integrative Medicine, 2017, 14, pp.28-31. ⟨10.1016/j.eujim.2017.08.009⟩ |
| ISSN: | 1876-3820 |
| Popis: | International audience; Introduction: Drugs resistant Plasmodium falciparum is a recurring issue that threatens public health. New anti-plasmodial drugs are needed to overcome this problem. The aim of this study was to characterize in vitro an-tiplasmodial activity of Withania frutescens (Solanaceae) against chloroquine-resistant Plasmodium falciparum strain. Methods: The in vitro antiplasmodial activity of leaves and roots extracts from Withania frutescens was performed in 96 well plates and preliminary phytochemical analysis was performed for the active fractions. The toxicity of the plant extract against CHO (Chinese Hamster Ovary) cells was assessed using the tetrazolium salt MTT col-orimetric assay. The selectivity index (SI) was calculated as the ratio between the cytotoxic and antiparasitic activities. Results: The methanol extract of W. frutescens leaves showed a good antiplasmodial activity (CC 50 18.1 μg/ml). Furthermore, ethyl acetate and butanol fractions showed promising in vitro antiplasmodial activity with a se-lectivity index of 3.1 and 2.4, respectively. The roots of W. frutescens were found to be inactive with a CC 50 value > 80 μg/ml. Conclusion: The antiplasmodial activity of W. frutescens may in part be attributed to the presence of polyphenolic and flavonoid compounds. Based on our results, ethyl acetate and butanol leaves fractions could be considered as a promising source for the development of putative antiplasmodial drugs. |
| Databáze: | OpenAIRE |
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