Glycoconjugates with Neu5Ac(alpha 2,6)Gal(beta 1,4)GlcNAc sequences: a selective lectin-histochemical property of Kurloff cells in guinea pig thymus
| Autor: | Slim-Eric Letaïef, N. Oulhaj, Gérard Landemore, Jacques Izard |
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| Rok vydání: | 1993 |
| Předmět: |
Male
Histology Kurloff cell Glycoconjugate Guinea Pigs Molecular Sequence Data Ribosome Inactivating Proteins Neuraminidase Thymus Gland Biology Sialidase Pathology and Forensic Medicine Agglutinin Lectins Animals chemistry.chemical_classification Histocytochemistry Acid phosphatase Lectin Cell Biology Oligosaccharide body regions Killer Cells Natural chemistry Biochemistry Carbohydrate Sequence biology.protein Female Plant Lectins Glycoprotein Glycoconjugates |
| Zdroj: | Cell and tissue research. 271(1) |
| ISSN: | 0302-766X |
| Popis: | The Kurloff cell (KC), a natural killer lymphocyte, contains a large (10-microns diameter) periodic acid-Schiff (PAS)-positive lysosome-like inclusion body called the Kurloff body (KB), which exhibits strong acid phosphatase activity. The presence of Sambucus nigra agglutinin (SNA)-reactive Neu5Ac(alpha 2,6)-D-Gal/Gal-NAc(beta 1,4)GlcNAc oligosaccharide sequences and the absence of the corresponding Neu5Ac(alpha 2,3) Maackia amurensis agglutinin (MAA)-reactive sequence in the major 35-kDa N-glycosylproteins of the complex or hybrid type extracted from purified KC were established by Western-lectin-blotting of cytosolic extracts from purified KC. Moreover, these SNA-reactive sequences, or at least part of them, were shown to be borne by sialidase-sensitive KC acid isophosphatases. Thymic sections rich in KC, from estrogenized guinea pigs were examined by affino-histochemistry with these sialic acid-reactive lectins. The SNA-reactivity of thymic sections was quasi-exclusively confined to KC clusters, whereas the whole thymic section was negative for MAA. KC were not SNA-reactive following preincubation and incubation with 200 mM lactose. When submitted to enzymatic or mild chemical desialylation processes, the SNA-reactivity of the KC clusters was enhanced. The SNA-reactivity of KC clusters was completely abolished following prolonged chemical desialylation, whereas the PAS-positivity of KB remained unchanged. Even after a prolonged sialidase treatment, this SNA-reactivity was only reduced. Moreover, after both these desialylation processes, KC developed a heavier Ricinus communis agglutinin-reactivity, thus confirming the presence of penultimate Gal residues in their abundant SNA-reactive oligosaccharide sequences Neu5Ac(alpha 2,6)Gal(beta 1,4)GlcNAc. Such a selective lectin histochemical property provides a marker for detecting KC. |
| Databáze: | OpenAIRE |
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