Leukaemia Inhibitory Factor or Related Factors Promote the Differentiation of Neuronal and Astrocytic Precursors within the Developing Murine Spinal Cord

Autor: Trevor J. Kilpatrick, David P. Gearing, Renée Dutton, Mark Murphy, Perry F. Bartlett, Seong-Seng Tan, Linda J. Richards
Rok vydání: 1996
Předmět:
Ornithine
Leukemia Inhibitory Factor Receptor alpha Subunit
Ciliary neurotrophic factor
Leukemia Inhibitory Factor
Biological Factors
Mice
Genes
Reporter
Cricetinae
Cells
Cultured
reproductive and urinary physiology
Neurons
Lymphokines
Mice
Inbred BALB C
Stem Cells
General Neuroscience
Antibodies
Monoclonal
Cell Differentiation
Growth Inhibitors
Cell biology
medicine.anatomical_structure
Spinal Cord
embryonic structures
Cell Division
hormones
hormone substitutes
and hormone antagonists
Neurotrophin
Astrocyte
endocrine system
Receptors
OSM-LIF
Mice
Transgenic
CHO Cells
Biology
Inhibitory postsynaptic potential
Astrocyte differentiation
Dosage Compensation
Genetic
medicine
Animals
Cell Lineage
Receptors
Cytokine
Interleukin-6
Fibroblasts
Embryonic stem cell
Coculture Techniques
Culture Media
Fibronectins
Astrocytes
Mice
Inbred CBA
biology.protein
Laminin
Neuron
Neuroscience
Leukemia inhibitory factor
Zdroj: European Journal of Neuroscience. 8:291-299
ISSN: 1460-9568
0953-816X
DOI: 10.1111/j.1460-9568.1996.tb01213.x
Popis: Previously we have shown that leukaemia inhibitory factor (LIF) potentiates the development of murine spinal cord neurons in vitro, suggesting that it, or related factors, may play an important regulatory role in neuronal development. We have further investigated this role and show here that the generation of neurons in cultures of embryonic day In spinal cord cells is inhibited by antibodies to the beta subunit of the LIF receptor. Since there are more undifferentiated precursors in antibody-treated cultures than in control and LIF-treated cultures, it is concluded that the primary action of LIF, or related molecules, is to promote neuronal differentiation, not precursor survival, In addition, the failure of LIF to support neuronal survival in the period immediately following differentiation suggests that the increased numbers of neurons generated with LIF are not attributable to its neurotrophic action. By selecting neuronal precursors on the basis of their inability to express class I major histocompatibility complex molecules, it was shown that LIF acted directly upon these cells and not via an intermediary cell. LIF also appears to be involved in regulating the differentiation of astrocytes, since it increases the number of glial fibrillary protein (GFAP)-positive cells present in the cultures and since the spontaneous production of GFAP-positive cells is blocked by antibodies to the LIF beta receptor. These findings suggest that LIF or related factors promote the differentiation of neural precursors in the spinal cord, but that they. are not involved in preferentially promoting precursors down a specific differentiation pathway.
Databáze: OpenAIRE
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