Activation patterns of murine B cells after oral administration of an encapsulated soluble antigen
| Autor: | J G Michael, Michael Flanagan, Shilpa L. Jain, K S Barone |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Ovalbumin
medicine.medical_treatment Administration Oral Capsules Biology Lymphocyte Activation Mice Peyer's Patches Immune system Th2 Cells Antigen Immunity Oral administration Intestine Small medicine Animals Intestinal Mucosa Immunity Mucosal B-Lymphocytes General Veterinary General Immunology and Microbiology Public Health Environmental and Occupational Health Isotype Molecular biology Small intestine Immunoglobulin A medicine.anatomical_structure Infectious Diseases Immunoglobulin G Immunology biology.protein Molecular Medicine Female Adjuvant |
| Zdroj: | Vaccine. 14(13) |
| ISSN: | 0264-410X |
| Popis: | Oral administration of soluble protein antigen, ovalbumin (OVA), encapsulated by an acid-resistant acrylic polymer, induced a strong immune response in BDF1 mice. Mice fed the encapsulated OVA showed activation of antigen-specific IgA, IgG and IgG1 isotype antibody-secreting cells which migrated to various lymphoid tissues via the common mucosal immune system traffic pathway. The highest number of antigen-specific antibody secreting cells was found in the Peyer's patches isolated from the upper segment of the small intestine, with their numbers declining along the length of the intestinal tract. Oral administration of this product suggests activation of lymphocytes exhibiting Th2 phenotype as shown by OVA-specific IgA and IgG1 isotype antibody responses. In conclusion, we demonstrated that oral administration of encapsulated soluble protein, in the absence of adjuvant, can activate the murine common mucosal immune system. |
| Databáze: | OpenAIRE |
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