Developmentally regulated telomere addition inTetrahymena thermophila
| Autor: | Elizabeth H. Blackburn, Elizabeth A. Spangler, Thecla Ryan |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
Cell Nucleus
Genetics Base Sequence Macronucleus Oligonucleotide Base pair Molecular Sequence Data Nucleotide Mapping Tetrahymena Nucleic Acid Hybridization Chromosome DNA Restriction Enzymes Biology biology.organism_classification Chromosomes Telomere Nucleic acid thermodynamics Genes Regulator Animals Cloning Molecular Repeated sequence |
| Zdroj: | Nucleic Acids Research. 16:5569-5585 |
| ISSN: | 1362-4962 0305-1048 |
| DOI: | 10.1093/nar/16.12.5569 |
| Popis: | To investigate the developmentally programmed telomere addition that accompanies chromosome fragmentation during macronuclear differentiation in Tetrahymena thermophila, five representative telomeric regions from the macronucleus were cloned and characterized in detail. The sequences adjacent to the telomeric (C4A2:T2G4) repeats on these five macronuclear ends had no significant sequence homology or shared secondary structure. Two developmentally independent examples of one macronuclear telomere had a 5 base pair difference in the position of the junction between the telomeric repeats and the adjacent sequences. A telomere-adjacent sequence, in the form of a synthetic oligonucleotide, was unable to prime the addition of telomeric repeats in vitro. The implications of these results for the mechanisms underlying developmentally programmed chromosome fragmentation and telomere addition in Tetrahymena are discussed. |
| Databáze: | OpenAIRE |
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