Vinclozolin modulates hepatic cytochrome P450 isoforms during pregnancy
| Autor: | Derly Constanza Escobar-Wilches, Ma. de Lourdes López-González, Adolfo Sierra-Santoyo, Roberto Chavira-Ramírez, Félix Genoveva García-Montes de Oca |
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| Rok vydání: | 2015 |
| Předmět: |
medicine.medical_specialty
Biology Toxicology Hydroxylation chemistry.chemical_compound Cytochrome P-450 Enzyme System Pregnancy Internal medicine medicine Animals Testosterone Vinclozolin Rats Wistar Receptor Oxazoles Estradiol Cytochrome P450 Androgen Antagonists Isoenzymes Endocrinology Liver chemistry Endocrine disruptor Toxicity biology.protein Female Hormone |
| Zdroj: | Reproductive Toxicology. 53:119-126 |
| ISSN: | 0890-6238 |
| Popis: | Vinclozolin (V) is classified as a potent endocrine disruptor. The aim of the present study was to determine the effects of V on rat liver CYP regulation and on serum levels of testosterone and estradiol during pregnancy. Pregnancy decreased the liver total CYP content by 65%, enzyme activities of MROD, PROD, and PNPH, and testosterone hydroxylation activities, as well as the protein content of CYP2A and 3A. V exposure remarkably induced the protein content and enzyme activities of CYP1A, 2A, 2B and 3A subfamilies. Testosterone and estradiol were affected in an opposite manner, provoking a 3.5-fold increase in the estradiol/testosterone ratio. These results suggest that V could regulate the hepatic CYP expression through interaction with receptors and coactivators involved in its expression and may play an important role in hormonal balance during pregnancy. In addition, the results may also contribute to understanding the toxicity of V by in utero exposure. |
| Databáze: | OpenAIRE |
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