Characterization of sea urchin egg endoplasmic reticulum in cortical preparations
| Autor: | Benjamin Kaminer, Mark Terasaki, Christian Sardet, John H. Henson, David A. Begg |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Calcium metabolism
biology Cortical endoplasmic reticulum Endoplasmic reticulum chemistry.chemical_element Cell Biology Anatomy Calcium Endoplasmic Reticulum biology.organism_classification Ribosome Strongylocentrotus purpuratus Staining Microscopy Electron chemistry Sea Urchins biology.animal Oocytes Biophysics Animals Molecular Biology Sea urchin Developmental Biology |
| Zdroj: | Developmental Biology. 148:398-401 |
| ISSN: | 0012-1606 |
| DOI: | 10.1016/0012-1606(91)90348-7 |
| Popis: | The cortical endoplasmic reticulum (ER) of sea urchin eggs was localized on isolated egg cortices by staining with aqueous suspensions of the dicarbocyanine “DiI.” Immunofluorescence localization of a calsequestrin-like protein was essentially identical; this is consistent with a role for the ER in calcium regulation. The ER often encircles cortical granules, making it well-suited for initiating fusion and propagating the calcium wave. Thiazole orange and Hoechst dye 33258 at pH 2 stain ribosomes bound to the ER, providing evidence that the cortical ER is rough ER. High chloride concentrations were found to disrupt ER continuity. |
| Databáze: | OpenAIRE |
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