Two copper-containing proteins from white and gray matter of brain
| Autor: | H. Ch. Buniatian, R.M. Nalbandyan, Svetlana Sharoyan, A.A. Shaljian |
|---|---|
| Rok vydání: | 1977 |
| Předmět: |
Amine oxidase
Copper protein chemistry.chemical_element Nerve Tissue Proteins Biochemistry Genetics and Molecular Biology (miscellaneous) Superoxide dismutase White matter Metalloproteins medicine Animals Amino Acids Brain Chemistry chemistry.chemical_classification Chromatography biology Superoxide Dismutase Electron Spin Resonance Spectroscopy Brain Copper Molecular Weight White (mutation) Enzyme Isoelectric point medicine.anatomical_structure chemistry Biochemistry Spectrophotometry biology.protein Cattle Spectrophotometry Ultraviolet |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Protein Structure. 493:478-487 |
| ISSN: | 0005-2795 |
| DOI: | 10.1016/0005-2795(77)90204-5 |
| Popis: | The procedure for the isolation of two water soluble copper-containing proteins from the white and gray matter of bovine brain is described. One of the proteins, cerebrocuprein I, is superoxide dismutase; and three molecular forms of this enzyme are to be found in brain. The other protein present in gray and white matter is devoid of superoxide dismutase and amine oxidase activities. The amino acid composition, molecular weight, isoelectric point and copper content of this protein were determined. The effect of some agents, pH and thermal treatment on the optical and EPR spectra of the protein were also studied. The copper of the protein may be removed and the holoprotein reconstituted again from apoprotein and copper. The results obtained led to the conclusion that in brain a new copper protein is discovered, which is named neurocuprein. |
| Databáze: | OpenAIRE |
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