Cyclin E and cyclin A are likely targets of Src for PDGF-induced DNA synthesis in fibroblasts
| Autor: | Gaël Manes, Serge Roche, Olivia Furstoss |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
DNA Replication
Cyclin E Cyclin D Cyclin A Biophysics Cyclin B Biochemistry Retinoblastoma Protein Mice Cyclin D1 Structural Biology Genetics Animals Molecular Biology Cyclin Mice Knockout Platelet-Derived Growth Factor biology DNA synthesis Chemistry E1A Cell Biology 3T3 Cells PDGF Fibroblasts Molecular biology Cell biology src-Family Kinases Gene Expression Regulation Cyclin-dependent kinase complex biology.protein Cyclin A2 Src |
| Zdroj: | FEBS letters. 526(1-3) |
| ISSN: | 0014-5793 |
| Popis: | How tyrosine kinases of the Src family regulate platelet-derived growth factor (PDGF)-induced DNA synthesis remains elusive. Here we show that the E1A antigen of adenovirus 5 overrides the G1 block elicited by the kinase-inactive mutant SrcK−. This was dependent upon the CR2 region of E1A that upregulated cyclin E and cyclin A and inactivated the pocket protein pRb. E1A rescue was independent of pRb. Expression of SrcK− in fibroblasts prevented PDGF-induced expression of cyclins E and A. This effect was overcome by E1A. Constitutive expression of cyclins E and A, but not D1, restored mitogenesis that was inhibited by SrcK−. We conclude that both cyclin E and cyclin A are likely targets of Src mediating PDGF-induced DNA synthesis. |
| Databáze: | OpenAIRE |
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