Biochemical and topological analysis of adult T-cell leukaemia-derived factor, homologous to thioredoxin, in the pregnant human uterus
| Autor: | Yuuki Kitaoka, Hiroshi Masutani, Fuminori Kobayashi, Norimasa Sagawa, Junji Yodoi, Shingo Fujii, Yoshihiko Nanbu, Takahide Mori, Hajime Nakamura |
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| Rok vydání: | 1995 |
| Předmět: |
Adult
Embryology Blotting Western Uterus Enzyme-Linked Immunosorbent Assay Pregnancy Proteins Biology Thioredoxins Pregnancy Placenta Decidua Genetics Homologous chromosome medicine Humans Amnion Molecular Biology Obstetrics and Gynecology Trophoblast Chorion Cell Biology T lymphocyte medicine.disease Immunohistochemistry Molecular biology Neoplasm Proteins Leukemia medicine.anatomical_structure Reproductive Medicine biology.protein Cytokines Female Antibody Thioredoxin Developmental Biology |
| Zdroj: | Molecular Human Reproduction. 1:203-208 |
| ISSN: | 1460-2407 1360-9947 |
| DOI: | 10.1093/molehr/1.4.203 |
| Popis: | Adult T-cell leukaemia-derived factor (ADF), homologous to thioredoxin, displays various biological activities, such as radical scavenging action and the reduction of protein disulphide bonds. We examined the biochemical and immunohistochemical localization of ADF in the pregnant human uterus, using two heteroantibodies to ADF, antibody C and W. Immunohistochemically, decidua and trophoblast cells were intensely stained by antibody C. The concentration of ADF-like substance in the decidua was 95.9 ng/mg protein, determined by enzyme-linked immunosorbent assay. The molecular weight of ADF-like substance in these tissues was determined by gel electrophoresis to be 13 kDa, the same as that of recombinant ADF. These findings indicate that abundant ADF is present in decidua and trophoblast cells; the localization of such a potent dithiol reducing substance may be beneficial in protecting the fertilized egg and placental trophoblasts from the cytotoxic effects of oxygen radicals. |
| Databáze: | OpenAIRE |
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