First mitochondrial and nuclear DNA sequences of Lamanema chavezi (Nematoda: Molineidae): Novel findings to improve its identification in feces from South American camelids
| Autor: | Romina Sandra Petrigh, María M. Cafrune, Martín H. Fugassa |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Mitochondrial DNA
Argentina Zoology DNA Mitochondrial Polymerase Chain Reaction DNA sequencing Feces Animals Parasite hosting Parasite Egg Count Ribosomal DNA Strongylida Infections Genome Helminth biology Nuclear Proteins Molineoidae Sequence Analysis DNA DNA Helminth biology.organism_classification Nuclear DNA Infectious Diseases Nematode Animals Domestic Female Parasitology Camelids New World Camelid |
| Zdroj: | Parasitology International. 68:60-62 |
| ISSN: | 1383-5769 |
| DOI: | 10.1016/j.parint.2018.10.007 |
| Popis: | Lamanema chavezi (Family Molineidae) is a parasitic nematode of South American camelids (SACs). A few studies have reported this parasite in SACs, mainly in domestic camelid species (llama and alpaca). Parasite identification by means of copro-parasitological methods is non-invasive and allows performing epidemiological studies. However, egg misidentification and difficulty to culture third-stage larvae do not allow identifying nematodes to species level. In contrast, molecular tools allow identifying eggs of gastrointestinal nematodes more accurately. However, the little genomic information available in databases for some species prevents an accurate diagnosis. In the present work, L. chavezi females present in feces of llamas from northwestern Argentina were molecularly characterized to obtain genomic information and improve parasitological diagnosis of L. chavezi-like eggs present in guanaco feces from southeastern Argentina. An 833-bp fragment of nuclear ribosomal DNA (rDNA) and a 434-bp fragment of the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene from both L. chavezi females and eggs were amplified and sequenced. Comparison between sequences from females and eggs showed 99–99.6% identity to rDNA and 99.5–96.1% to the cox1 gene fragments, confirming egg morphological assignment. A higher divergence between sequences was observed in the cox1 fragment, with a maximum variation of 3.9%. The examination of eggs found in guanaco feces from southeastern Argentina and their specific molecular identification represent the first record for this host in Argentine Patagonia and contribute to improving the diagnosis of gastrointestinal nematodes in SACs, mainly in wild camelids. |
| Databáze: | OpenAIRE |
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