Optimization of pectinase-assisted and tri-solvent-mediated extraction and recovery of lycopene from waste tomato peels
| Autor: | Vishal R. Parate, Abhijeet B. Muley, Amesh V. Pawar, Mayur R. Ladole, Pravin J. Munde, Mohammed I. Talib |
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| Rok vydání: | 2017 |
| Předmět: |
Chromatography
ABTS DPPH Extraction (chemistry) 04 agricultural and veterinary sciences 02 engineering and technology Environmental Science (miscellaneous) 021001 nanoscience & nanotechnology Ascorbic acid 040401 food science Agricultural and Biological Sciences (miscellaneous) High-performance liquid chromatography Lycopene Solvent chemistry.chemical_compound Horticulture 0404 agricultural biotechnology chemistry Original Article Pectinase 0210 nano-technology Biotechnology |
| Zdroj: | 3 Biotech. 7 |
| ISSN: | 2190-5738 2190-572X |
| DOI: | 10.1007/s13205-017-0825-3 |
| Popis: | In the present work, optimization of pectinase-assisted and tri-solvent-mediated extraction of lycopene from waste tomato peels was carried out. The optimized parameters for enzymatic pre-treatment were 2% pectinase concentration, pH 5.5, 4-h incubation, 45 °C and 150 rpm. Maximum recovery of lycopene from tomato peels using optimized tri-solvent extraction was achieved at 45 °C, 120-min incubation and 200 rpm. The extracted lycopene was confirmed through functional and characteristic peaks in UV–Vis and FTIR spectra and with retention time in HPLC. The radical scavenging activity was 72.30 ± 2.70 and 43.40 ± 2.01 µg ascorbic acid equivalents (AAE)/ml for 1,1-diphenyl-2-picrylhydrzyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radicals, respectively. The optimized method resulted in 7.38, 4.65 and 1.59 times enhancement in lycopene extraction and recovery in correlation with single solvent, enzyme-treated and tri-solvent extraction methods, respectively. |
| Databáze: | OpenAIRE |
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