Frequency of CCR5 Delta-32 Mutation in Human Immunodeficiency Virus (HIV)-seropositive and HIV-exposed Seronegative Individuals and in General Population of Medellin, Colombia
| Autor: | María Teresa Rugeles, Cecilia Villegas, Rodrigo Vesga, Jorge Vega, Jorge Nagles, Gabriel Bedoya, Pablo J. Patiño, Francisco J. Díaz |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2000 |
| Předmět: |
Adult
Male Microbiology (medical) lcsh:Arctic medicine. Tropical medicine Genotype Receptors CCR5 lcsh:RC955-962 Chemokine receptor CCR5 Population lcsh:QR1-502 Receptores CCR5 HIV Infections Colombia ∆32 mutation Polymerase Chain Reaction lcsh:Microbiology law.invention human immunodeficiency virus (HIV) infection Gene Frequency law HIV Seronegativity HIV Seropositivity Humans Allele Seroconversion education Gene Alleles Polymerase chain reaction Aged education.field_of_study Chi-Square Distribution biology HIV VIH Middle Aged Virology Delta-32 mutation exposed seronegative Mutation Immunology biology.protein Female CCR5 |
| Zdroj: | Memórias do Instituto Oswaldo Cruz, Volume: 95, Issue: 2, Pages: 237-242, Published: APR 2000 Repositorio UdeA Universidad de Antioquia instacron:Universidad de Antioquia Memórias do Instituto Oswaldo Cruz., Vol 95, Iss 2, Pp 237-242 (2000) |
| Popis: | Repeated exposure to human immunodeficiency virus (HIV) does not always result in seroconversion. Modifications in coreceptors for HIV entrance to target cells are one of the factors that block the infection. We studied the frequency of ∆32 mutation in ccr5 gene in Medellin, Colombia. Two hundred and eighteen individuals distributed in three different groups were analyzed for ∆32 mutation in ccr5 gene by polymerase chain reaction (PCR): 29 HIV seropositive (SP), 39 exposed seronegative (ESN) and 150 individuals as a general population sample (GPS). The frequency of the ∆32 mutant allele was 3.8% for ESN, 2.7% for GPS and 1.7% for SP. Only one homozygous mutant genotype (∆32/∆32) was found among the ESN (2.6%). The heterozygous genotype (ccr5/∆32) was found in eight GPS (5.3%), in one SP (3.4%) and in one ESN (2.6%). The differences in the allelic and genotypic frequencies among the three groups were not statistically significant. A comparison between the expected and the observed genotypic frequencies showed that these frequencies were significantly different for the ESN group, which indirectly suggests a protective effect of the mutant genotype (∆32/∆32). Since this mutant genotype explained the resistance of infection in only one of our ESN persons, different mechanisms of protection must be playing a more important role in this population. COL0012444 COL0006723 COL0012426 |
| Databáze: | OpenAIRE |
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