Serum N-glycan profiles differ for various breast cancer subtypes
| Autor: | Yuri E. M. van der Burgt, Gerda C M Vreeker, Manfred Wuhrer, Marco R. Bladergroen, Wilma E. Mesker, Simone Nicolardi, Kiki M.H. Vangangelt, Rob A. E. M. Tollenaar |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Oncology
medicine.medical_specialty Glycan Tumor heterogeneity Population Breast Neoplasms Disease Biochemistry 03 medical and health sciences 0302 clinical medicine Breast cancer Polysaccharides Internal medicine medicine Biomarkers Tumor Mammography Humans education Molecular Biology Glycomics 030304 developmental biology Aged Aged 80 and over 0303 health sciences education.field_of_study medicine.diagnostic_test biology Mass spectrometry business.industry Cancer Cell Biology Middle Aged medicine.disease Serum protein glycosylation 030220 oncology & carcinogenesis Case-Control Studies Cohort biology.protein Biomarker (medicine) Original Article Female Glycan profiling business |
| Zdroj: | Glycoconjugate Journal, 38(3), 387-395. SPRINGER Glycoconjugate Journal |
| Popis: | Breast cancer is the most prevalent cancer in women. Early detection of this disease improves survival and therefore population screenings, based on mammography, are performed. However, the sensitivity of this screening modality is not optimal and new screening methods, such as blood tests, are being explored. Most of the analyses that aim for early detection focus on proteins in the bloodstream. In this study, the biomarker potential of total serum N-glycosylation analysis was explored with regard to detection of breast cancer. In an age-matched case-control setup serum protein N-glycan profiles from 145 breast cancer patients were compared to those from 171 healthy individuals. N-glycans were enzymatically released, chemically derivatized to preserve linkage-specificity of sialic acids and characterized by high resolution mass spectrometry. Logistic regression analysis was used to evaluate associations of specific N-glycan structures as well as N-glycosylation traits with breast cancer. In a case-control comparison three associations were found, namely a lower level of a two triantennary glycans and a higher level of one tetraantennary glycan in cancer patients. Of note, various other N-glycomic signatures that had previously been reported were not replicated in the current cohort. It was further evaluated whether the lack of replication of breast cancer N-glycomic signatures could be partly explained by the heterogenous character of the disease since the studies performed so far were based on cohorts that included diverging subtypes in different numbers. It was found that serum N-glycan profiles differed for the various cancer subtypes that were analyzed in this study. |
| Databáze: | OpenAIRE |
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