IN VITRO RELEASE OF LUTEINIZING HORMONE-RELEASING HORMONE (LHRH) FROM RAT MEDIOBASAL HYPOTHALAMUS: EFFECTS OF POTASSIUM, CALCIUM AND DOPAMINE
| Autor: | Jacques Epelbaum, Claude Kordon, Jean-Louis Charli, E. Pattou, W.H. Rotsztejn |
|---|---|
| Rok vydání: | 1976 |
| Předmět: |
Male
endocrine system medicine.medical_specialty Dopamine Hypothalamus Hypothalamus Middle chemistry.chemical_element Peptide Gonadotropin-releasing hormone In Vitro Techniques Calcium Biology Gonadotropin-Releasing Hormone Bacitracin Endocrinology Internal medicine medicine Animals chemistry.chemical_classification Estradiol Ovary Pituitary Hormone-Releasing Hormones Rats chemistry Potassium Ovariectomized rat Female Luteinizing hormone hormones hormone substitutes and hormone antagonists medicine.drug |
| Zdroj: | Endocrinology. 99:1663-1666 |
| ISSN: | 1945-7170 0013-7227 |
| DOI: | 10.1210/endo-99-6-1663 |
| Popis: | A suitable preparation to study the effect of dopamine (DA) on in. vitro LHRH secretion is presented. Enzymatic degradation of LHRH in the incubation medium is completely inhibited by bacitracin (2 × 10−5 M). A high potassium concentration (56mM) induces an increase in LHRH release from entire pieces of mediobasal hypothalamus (MBH), but not from a synaptosomal pellet; this release is inhibited when calcium is omitted. The depolarization-induced LHRH release in vitro is independent of the initial amount of the peptide present in the MBH. In contrast, DA (10−6M) is not effective in vitro on MBH from ovariectomized rats but induces a fast release of LHRH from MBH of normal male and estradiol-pretreated ovariectomized rats. The preparation presented here appears to be of great interest in investigating amine-steroid-LHRH interactions at the cellular level. |
| Databáze: | OpenAIRE |
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