Benzo(a)pyrene Metabolism and EROD and GST Biotransformation Activity in the Liver of Red- and White-Blooded Antarctic Fish
Autor: | Peter Schmid, Helmut Segner, Anneli Strobel, Patricia Burkhardt-Holm |
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Rok vydání: | 2015 |
Předmět: |
Male
Acclimatization Climate Change Antarctic Regions Biology Chaenocephalus aceratus Xenobiotics Notothenia rossii chemistry.chemical_compound Benzo(a)pyrene Cytochrome P-450 CYP1A1 Animals Environmental Chemistry 14. Life underwater Glutathione Transferase Champsocephalus General Chemistry biology.organism_classification Adaptation Physiological Perciformes Cold Temperature Liver chemistry 13. Climate action Oncorhynchus mykiss Bioaccumulation Environmental chemistry Inactivation Metabolic Female Rainbow trout Xenobiotic Clearance rate Water Pollutants Chemical |
Zdroj: | Environmental Science & Technology. 49:8022-8032 |
ISSN: | 1520-5851 0013-936X |
Popis: | Climate change and anthropogenic pollution are of increasing concern in remote areas such as Antarctica. The evolutionary adaptation of Antarctic notothenioid fish to the cold and stable Southern Ocean led to a low plasticity of their physiological functions, what may limit their capacity to deal with altered temperature regimes and pollution in the Antarctic environment. Using a biochemical approach, we aimed to assess the hepatic biotransformation capacities of Antarctic fish species by determining (i) the activities of ethoxyresorufin-O-deethylase (EROD) and glutathione-S-transferase (GST), and (ii) the metabolic clearance of benzo(a)pyrene by hepatic S9 supernatants. In addition, we determined the thermal sensitivity of the xenobiotic biotransformation enzymes. We investigated the xenobiotic metabolism of the red-blooded Gobionotothen gibberifrons and Notothenia rossii, the hemoglobin-less Chaenocephalus aceratus and Champsocephalus gunnari, and the rainbow trout Oncorhynchus mykiss as a reference. Our results revealed similar metabolic enzyme activities and metabolic clearance rates between red- and white-blooded Antarctic fish, but significantly lower rates in comparison to rainbow trout. Therefore, bioaccumulation factors for metabolizable lipophilic contaminants may be higher in Antarctic than in temperate fish. Likewise, the thermal adaptive capacities and flexibilities of the EROD and GST activities in Antarctic fish were significantly lower than in rainbow trout. As a consequence, increasing water temperatures in the Southern Ocean will additionally compromise the already low detoxification capacities of Antarctic fish. |
Databáze: | OpenAIRE |
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