DIOXYGENASE FOR AUXIN OXIDATION 1 catalyzes the oxidation of IAA amino acid conjugates
| Autor: | Aleš Pěnčík, Petre I. Dobrev, Ondřej Novák, Karel Harant, Federica Brunoni, Katarzyna Retzer, Tomas Moravec, Klára Hoyerová, Kateřina Malínská, Karel Müller, Roberta Filepová, Zuzana Vondráková, Jan Petrášek, Lenka Helusová, Petr Hosek |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Physiology Nicotiana tabacum Mutant Plant Science 01 natural sciences Dioxygenases Transcriptome 03 medical and health sciences Auxin Arabidopsis Tobacco Genetics Arabidopsis thaliana heterocyclic compounds Amino Acids Research Articles Plant Proteins chemistry.chemical_classification biology Chemistry fungi food and beverages Plant cell biology.organism_classification Amino acid 030104 developmental biology Biochemistry Oxidation-Reduction 010606 plant biology & botany |
| Zdroj: | Plant Physiol |
| Popis: | Together with auxin transport, auxin metabolism is a key determinant of auxin signaling output by plant cells. Enzymatic machinery involved in auxin metabolism is subject to regulation based on numerous inputs, including the concentration of auxin itself. Therefore, experiments characterizing altered auxin availability and subsequent changes in auxin metabolism could elucidate the function and regulatory role of individual elements in the auxin metabolic machinery. Here, we studied auxin metabolism in auxin-dependent tobacco BY-2 cells. We revealed that the concentration of N-(2-oxindole-3-acetyl)-l-aspartic acid (oxIAA-Asp), the most abundant auxin metabolite produced in the control culture, dramatically decreased in auxin-starved BY-2 cells. Analysis of the transcriptome and proteome in auxin-starved cells uncovered significant downregulation of all tobacco (Nicotiana tabacum) homologs of Arabidopsis (Arabidopsis thaliana) DIOXYGENASE FOR AUXIN OXIDATION 1 (DAO1), at both transcript and protein levels. Auxin metabolism profiling in BY-2 mutants carrying either siRNA-silenced or CRISPR-Cas9-mutated NtDAO1, as well as in dao1-1 Arabidopsis plants, showed not only the expected lower levels of oxIAA, but also significantly lower abundance of oxIAA-Asp. Finally, ability of DAO1 to oxidize IAA-Asp was confirmed by an enzyme assay in AtDAO1-producing bacterial culture. Our results thus represent direct evidence of DAO1 activity on IAA amino acid conjugates. |
| Databáze: | OpenAIRE |
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