Hyaluronan and versican in the control of human T-lymphocyte adhesion and migration
| Autor: | Stephen P. Evanko, Susan Potter-Perigo, Paul L. Bollyky, Thomas N. Wight, Gerald T. Nepom |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
CD4-Positive T-Lymphocytes
Hyaluronoglucosaminidase Inflammation Plasma protein binding Lymphocyte Activation Time-Lapse Imaging Article Extracellular matrix Versicans Cell Movement Hyaluronidase Cell Adhesion medicine Humans Hyaluronic Acid Cell adhesion Lung Molecular Biology Cells Cultured biology Chemistry T lymphocyte Fibroblasts Extracellular Matrix Cell biology carbohydrates (lipids) Hyaluronan Receptors Poly I-C Immunology biology.protein Versican Collagen medicine.symptom Myofibroblast Fluorescein-5-isothiocyanate Protein Binding medicine.drug |
| Zdroj: | Matrix Biology. 31:90-100 |
| ISSN: | 0945-053X |
| DOI: | 10.1016/j.matbio.2011.10.004 |
| Popis: | The ability of lymphocytes to migrate freely through connective tissues is vital to efficient immune function. How the extracellular matrix (ECM) may affect T cell adhesion and migration is not well understood. We have examined the adhesion and migration of activated human T lymphocytes on extracellular matrix made by fibroblast-like synoviocytes and lung fibroblasts. These cells were minimally interactive until treated with a viral mimetic, Poly I:C. This treatment promoted myofibroblast formation and engendered a higher-order structured ECM rich in versican and hyaluronan to which T cells avidly adhered in a hyaluronidase-sensitive manner. This Poly I:C-induced matrix impeded T-cell spreading and migration on and through synoviocyte monolayers, while hyaluronidase treatment or adding versican antibody during matrix formation reversed the effect on T-cell migration. Hyaluronidase also reversed the spread myofibroblast morphology. These data suggest that the viscous hyaluronan- and versican-rich matrix binds and constrains T lymphocytes. Using purified matrix components and solid state matrices of defined composition we uncovered a role for versican in modulating HA - T-cell interactions. Versican prevented T-cell binding to soluble hyaluronan, as well as the amoeboid shape change on hyaluronan coated dishes and T-cell penetration of collagen gels. Together, these data suggest that hyaluronan and versican play a role in T-cell trafficking and function in inflamed tissues. |
| Databáze: | OpenAIRE |
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