Long non-coding RNA AK058003, as a precursor of miR-15a, interacts with HuR to inhibit the expression of γ-synuclein in hepatocellular carcinoma cells
| Autor: | Huilin Liang, Jun-Jian Deng, Yongfa Zheng, Xiaoqin He, Dongcheng Wu, Yuan-Yuan Gan, Ximing Xu, Yuefeng Zhang, Wei Ge, Yujie Zhou |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Carcinoma Hepatocellular Time Factors tumor suppressor Down-Regulation Kaplan-Meier Estimate Bioinformatics ELAV-Like Protein 1 law.invention Metastasis liver cancer 03 medical and health sciences gamma-Synuclein lncRNA Cell Movement law Databases Genetic Humans Medicine Neoplasm Invasiveness mRNA stabilizing protein Cell Proliferation Proportional Hazards Models Messenger RNA business.industry Cell growth Liver Neoplasms Computational Biology Cancer Hep G2 Cells Middle Aged medicine.disease Long non-coding RNA Neoplasm Proteins Gene Expression Regulation Neoplastic MicroRNAs 030104 developmental biology Oncology Hepatocellular carcinoma Cancer research Suppressor Female RNA Long Noncoding business Liver cancer Signal Transduction Research Paper |
| Zdroj: | Oncotarget |
| ISSN: | 1949-2553 |
| DOI: | 10.18632/oncotarget.14276 |
| Popis: | // Xiaoqin He 1, * , Yongfa Zheng 1, * , Yuefeng Zhang 2 , Yuanyuan Gan 1 , Yujie Zhou 1 , Huilin Liang 1 , Dongcheng Wu 3 , Wei Ge 1 , Junjian Deng 1 , Ximing Xu 1 1 Cancer Center, Renmin Hospital of Wuhan University, Wuhan, China 2 Department of Surgery, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China 3 Department of Biochemistry and Molecular Chemistry, School of Basic Medicial Sciences, Wuhan University, Wuhan, China * These authors have contributed equally to this work Correspondence to: Ximing Xu, email: doctorxu120@aliyun.com Keywords: liver cancer, tumor suppressor, lncRNA, mRNA stabilizing protein Received: October 07, 2016 Accepted: December 15, 2016 Published: December 27, 2016 ABSTRACT Long non-coding RNAs (lncRNAs) have been identified as critical players in multiple cancers and lncRNAs are tightly linked to cancer progression. However, only little amount of lncRNAs have been identified to participate in the molecular mechanisms of the progression of hepatocellular carcinoma. In this study, we found that lncRNA-AK058003 is down-regulated in hepatocellular carcinoma tissues and it is associated with the relapse and metastasis of the cancer. Furthermore, lncRNA-AK058003 acts as a tumor suppressor, suppressing hepatocellular carcinoma cell proliferation and metastasis in vitro and in vivo . lncRNA-AK058003 can reduce mRNA stabilizing protein HuR, which results in the inhibition of the expression of γ-synuclein. In addition, a bioinformatics study indicated that γ-synuclein is a target of miR-15a. To verify whether lncRNA-AK058003 plays a role in miR-15a-mediated inhibition of γ-synuclein, we demonstrated that lncRNA-AK058003 is very likely to be a precursor of miR-15a. Collectively, lncRNA-AK058003 can reduce the expression of mRNA stabilizing protein HuR and act as a precursor of miR-15a to suppress γ-synuclein-mediated cell proliferation and the metastasis of hepatocellular carcinoma. |
| Databáze: | OpenAIRE |
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