Chromatographic enantioseparation of amino acids using a new chiral stationary phase based on a macrocyclic glycopeptide antibiotic
Autor: | Sergey M. Staroverov, K. Gedicke, Vera Meshko, Mikhail A. Kuznetsov, Andreas Seidel-Morgenstern, K. Petrusevska |
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Rok vydání: | 2006 |
Předmět: |
Macrocyclic Compounds
medicine.drug_class Filtration and Separation Glycopeptide antibiotic High-performance liquid chromatography Analytical Chemistry symbols.namesake Adsorption Phase (matter) parasitic diseases Materials Testing medicine Amino Acids Chromatography High Pressure Liquid Chromatography Molecular Structure Teicoplanin Elution Chemistry fungi Glycopeptides Langmuir adsorption model Stereoisomerism Anti-Bacterial Agents symbols Enantiomer Porosity Mathematics medicine.drug |
Zdroj: | Journal of separation science. 29(10) |
ISSN: | 1615-9306 |
Popis: | The separation of the enantiomers of several a-amino acids was studied on a new chiral stationary phase (CSP) which is based on the macrocyclic glycopeptide antibiotic eremomycin attached to silica particles. Retention and separation factors were determined under analytical conditions at ambient temperature for different mobile phase compositions. In order to evaluate the potential with respect to preparative separations the adsorption isotherms of D- and L-methionine were determined for one mobile phase composition applying the elution by characteristic point method. The isotherms were validated by comparing experimentally determined elution profiles with predictions based on the equilibrium dispersive model. Finally, the performance of the eremomycin CSP was compared with a commercially available CSP based on the macrocyclic antibiotic teicoplanin. After determining the isotherms of D- and L-methionine also for the teicoplanin phase, the equilibrium dispersive model was used for both CSP to identify optimal operating conditions. For the separation and conditions considered the new eremomycin CSP revealed a better performance compared to the teicoplanin CSP. |
Databáze: | OpenAIRE |
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