A new method to measure iron absorption from the enrichment of 57Fe and 58Fe in young erythroid cells
| Autor: | Gertjan Schaafsma, Theo Muys, Steven Spanhaak, Hillie Pellegrom, Joost P. Bruyntjes, Ellen G. H. M. van den Heuvel, Wim van Dokkum |
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| Přispěvatelé: | Humane Biologie, RS: NUTRIM School of Nutrition and Translational Research in Metabolism, Centraal Instituut voor Voedingsonderzoek TNO |
| Jazyk: | angličtina |
| Rok vydání: | 1998 |
| Předmět: |
Adult
Absorption (pharmacology) Erythrocytes Reticulocytes Iron Iron absorption Clinical Biochemistry Administration Oral Iron kinetics Cell Separation Mass spectrometry Mass Spectrometry Absorption Oral administration Humans Nutrition Whole blood Chromatography Isotope Chemistry Stable isotope ratio Biochemistry (medical) Radioisotope distribution Erythrocyte Aging Iron Isotopes Centrifugation Zonal Normal human Cell membrane transport Erythroid cell Human cell Isotope Labeling Ferritins Injections Intravenous Female Controlled study Quantitative analysis (chemistry) Human |
| Zdroj: | Clinical Chemistry, 44(3), 649-654. American Association for Clinical Chemistry Clinical Chemistry, 3, 44, 649-654 Europe PubMed Central |
| ISSN: | 1530-8561 0009-9147 |
| Popis: | TNO Nutrition and Food Research Institute, AJ Zeist, The Netherlands.Iron absorption can be measured by the incorporation of stable iron isotopes into erythrocytes, 14 days after isotope administration. The disadvantage of this method is the high dose of isotopes needed to obtain a sufficient enrichment. Therefore, in this study cell fractions rich in young erythroid cells were prepared by using a density separation method. From 10 women blood was taken 4, 5, and 7 days after oral and intravenous administration of 57Fe and 58Fe. In these cell fractions and in whole blood taken 14 days after isotope administration, isotope enrichment was measured and absorption calculated. Absorption calculated from the isotope enrichment in the reticulocyte-rich cell fractions (12.2 +/- SEM 3.7%) was not significantly different from absorption based on whole-blood values (13.0 +/- 3.3%). Because a threefold higher isotope enrichment was found in the cell fractions, the required dose of stable isotopes can be reduced to one-third of the dose used in the traditional method without loss of sensitivity. |
| Databáze: | OpenAIRE |
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