Peroxisome biogenesis and human peroxisome-deficiency disorders
| Autor: | Yukio Fujiki |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
FIS1
Peroxisome membrane biogenesis General Physics and Astronomy CHO Cells Review Biology Peroxisomal Disorders Cricetulus Protein-fragment complementation assay Cricetinae Peroxisomes peroxins Zellweger syndrome medicine Animals Humans peroxisome biogenesis Chinese hamster ovary cell General Medicine Peroxisome medicine.disease pathogenic genes CHO cell mutants Cell biology Complementation Biochemistry protein import machinery General Agricultural and Biological Sciences Biogenesis |
| Zdroj: | Proceedings of the Japan Academy. Series B, Physical and Biological Sciences |
| ISSN: | 1349-2896 0386-2208 |
| DOI: | 10.2183/pjab.92.463 |
| Popis: | Peroxisome is a single-membrane-bounded ubiquitous organelle containing a hundred different enzymes that catalyze various metabolic pathways such as β-oxidation of very long-chain fatty acids and synthesis of plasmalogens. To investigate peroxisome biogenesis and human peroxisome biogenesis disorders (PBDs) including Zellweger syndrome, more than a dozen different complementation groups of Chinese hamster ovary (CHO) cell mutants impaired in peroxisome biogenesis are isolated as a model experimental system. By taking advantage of rapid functional complementation assay of the CHO cell mutants, successful cloning of PEX genes encoding peroxins required for peroxisome assembly invaluably contributed to the accomplishment of cloning of pathogenic genes responsible for PBDs. Peroxins are divided into three groups: 1) peroxins including Pex3p, Pex16p and Pex19p, are responsible for peroxisome membrane biogenesis via Pex19p- and Pex3p-dependent class I and Pex19p- and Pex16p-dependent class II pathways; 2) peroxins that function in matrix protein import; 3) those such as Pex11pβ are involved in peroxisome division where DLP1, Mff, and Fis1 coordinately function. |
| Databáze: | OpenAIRE |
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