Associations of single nucleotide polymorphisms in precursor-microRNA (miR)-125a and the expression of mature miR-125a with the development and prognosis of autoimmune thyroid diseases

Autor:	S. Shibutani, Hiroshi Otsu, Yuka Inoue, Yukina Takuse, Yoshinori Iwatani, T. Kagawa, Mikio Watanabe, Naoya Inoue, Minori Saeki, Yoh Hidaka
Rok vydání:	2014
Předmět:	Untranslated region Adult Male Adolescent Genotype T cell Immunology Gene Expression Single-nucleotide polymorphism Hashimoto Disease Biology Peripheral blood mononuclear cell Polymorphism Single Nucleotide Young Adult Immune system Gene Frequency microRNA medicine RNA Precursors Immunology and Allergy Humans Genetic Predisposition to Disease Child Alleles Genetic Association Studies Aged Aged 80 and over Thyroiditis Autoimmune Interleukin Original Articles Middle Aged Prognosis Graves Disease MicroRNAs medicine.anatomical_structure Gene Expression Regulation Case-Control Studies Leukocytes Mononuclear Female
Zdroj:	Clinical and experimental immunology. 178(2)
ISSN:	1365-2249
Popis:	Summary It is important to search the biomarker to predict the development and prognosis of autoimmune thyroid diseases (AITDs) such as Hashimoto's disease (HD) and Graves' disease (GD). MicroRNA (miR) bind directly to the 3′ untranslated region of specific target mRNAs to suppress the expression of proteins, promote the degradation of target mRNAs and regulate immune response. miR-125a is known to be a negative regulator of regulated upon activation normal T cell expressed and secreted (RANTES), interleukin (IL)-6 and transforming growth factor (TGF)-β; however, its association with AITDs remains unknown. To clarify the association between AITDs and miR-125a, we genotyped the rs12976445 C/T, rs10404453 A/G and rs12975333 G/T polymorphisms in the MIR125A gene, which encodes miR-125a, using direct sequencing and polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) methods in 155 patients with GD, 151 patients with HD and 118 healthy volunteers. We also examined the expression of miR-125a in peripheral blood mononuclear cells (PBMCs) from 55 patients with GD, 79 patients with HD and 38 healthy volunteers using quantitative real-time PCR methods. We determined that the CC genotype and C allele of the rs12976445 C/T polymorphism were significantly more frequent in patients with HD compared with control subjects (P < 0·05) and in intractable GD compared with GD in remission (P < 0·05). The expression of miR-125a was correlated negatively with age (P = 0·0010) and down-regulated in patients with GD compared with control subjects (P = 0.0249). In conclusion, miR-125a expression in PBMCs and the rs12976445 C/T polymorphism were associated with AITD development and prognosis.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9098f61beea2c25dd1e53530af717412 https://pubmed.ncbi.nlm.nih.gov/24990808 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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