Sulforaphane induces Nrf2 target genes and attenuates inflammatory gene expression in microglia from brain of young adult and aged mice

Autor: Rodney W. Johnson, Brigitte E. Townsend
Rok vydání: 2016
Předmět:
Lipopolysaccharides
Male
0301 basic medicine
Aging
Lipopolysaccharide
NF-E2-Related Factor 2
Drug Evaluation
Preclinical
Pharmacology
medicine.disease_cause
Biochemistry
Article
Proinflammatory cytokine
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Endocrinology
Isothiocyanates
Genetics
medicine
Animals
Molecular Biology
Cells
Cultured
Neuroinflammation
Mice
Inbred BALB C
Microglia
biology
business.industry
Anti-Inflammatory Agents
Non-Steroidal
Interleukin
Cell Biology
Up-Regulation
DNA-Binding Proteins
Nitric oxide synthase
030104 developmental biology
medicine.anatomical_structure
Gene Expression Regulation
chemistry
Sulfoxides
Immunology
biology.protein
Cytokines
Inflammation Mediators
business
030217 neurology & neurosurgery
Oxidative stress
Sulforaphane
Zdroj: Experimental Gerontology. 73:42-48
ISSN: 0531-5565
DOI: 10.1016/j.exger.2015.11.004
Popis: Increased neuroinflammation and oxidative stress resulting from heightened microglial activation are associated with age-related cognitive impairment. The objectives of this study were to examine the effects of the bioactive sulforaphane (SFN) on the nuclear factor E2-related factor 2 (Nrf2) pathway in BV2 microglia and primary microglia, and to evaluate proinflammatory cytokine expression in lipopolysaccharide (LPS)-stimulated primary microglia from adult and aged mice. BV2 microglia and primary microglia isolated from young adult and aged mice were treated with SFN and LPS. Changes in Nrf2 activity, expression of Nrf2 target genes, and levels of proinflammatory markers were assessed by quantitative PCR and immunoassay. SFN increased Nrf2 DNA-binding activity and upregulated Nrf2 target genes in BV2 microglia, while reducing LPS-induced interleukin (IL-)1β, IL-6, and inducible nitric oxide synthase (iNOS). In primary microglia from adult and aged mice, SFN increased expression of Nrf2 target genes and attenuated IL-1β, IL-6, and iNOS induced by LPS. These data indicate that SFN is a potential beneficial supplement that may be useful for reducing microglial mediated neuroinflammation and oxidative stress associated with aging.
Databáze: OpenAIRE
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