Cytotoxicity and Genotoxicity of Cationic Phosphorus-Containing Dendrimers
| Autor: | Anne-Marie Caminade, Janusz Blasiak, B. Klajnert, Paulina Gomulak, Maria Bryszewska, Jean-Pierre Majoral |
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| Přispěvatelé: | Łódź University of Technology, Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, University of Lódź, Laboratoire de chimie de coordination (LCC), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie de Toulouse (ICT-FR 2599), Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Institut de Recherche pour le Développement (IRD)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Cell viability
DNA damage Population education Apoptosis 02 engineering and technology Biology Cell cycle medicine.disease_cause 010402 general chemistry 01 natural sciences Biochemistry Human lymphocytes Drug Discovery medicine A549 cells [CHIM.COOR]Chemical Sciences/Coordination chemistry Viability assay Cytotoxicity Comet assay A549 cell Pharmacology education.field_of_study Morphology assay Plasmid relaxation assay Organic Chemistry 021001 nanoscience & nanotechnology Molecular biology 0104 chemical sciences HGF cells Molecular Medicine 0210 nano-technology Genotoxicity Cationic phosphorus-containing dendrimer |
| Zdroj: | Current Medicinal Chemistry Current Medicinal Chemistry, Bentham Science Publishers, 2012, 19 (36), pp.6233-6240. ⟨10.2174/092986712804485809⟩ ResearcherID |
| ISSN: | 0929-8673 |
| DOI: | 10.2174/092986712804485809⟩ |
| Popis: | International audience; Cationic phosphorus-containing dendrimers (CPDs) are a class of highly-branched polymers with potential medical relevance. However, little is known about CPD modes of interactions with cell and its components, including DNA. In the present work we investigated cytotoxicity and genotoxicity of CPDs generation 3 and 4 (CPD G3 and CPD G4) in human mononuclear blood cells, A549 human cancer cells and human gingival fibroblasts (HGFs). CPD G3 and CPD G4 at concentrations up to 10 μM induced a concentrationdependent decrease in cell viability as assessed by flow cytometry. Both compounds did not induce breaks in isolated DNA as evaluated by the plasmid relaxation assay but they induced DNA cross-links in the cells, as examined by comet assay. CPD G3 and 4 induced slight perturbations in the cell cycle leading to a decrease in the G2/M cell population accompanied by an increase in the S cell population. Upon treatment with CPDs, the cells showed changes in their morphology, including loss of cell attachment, disruption of cell membrane and nucleus condensation. Our results indicate that CPD G3 and G4 are cytotoxic and genotoxic for the assorted human cells. Therefore, CPDs may form stable complexes with DNA and interfere with cellular processes. |
| Databáze: | OpenAIRE |
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