Effects of chlorpyrifos on the growth and ultrastructure of green algae, Ankistrodesmus gracilis
| Autor: | Carolina Fernández, Yolanda Zalocar, Viviana Magdalena Asselborn, Elisa R. Parodi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Insecticides
Health Toxicology and Mutagenesis TOXICITY MICROALGAE purl.org/becyt/ford/1 [https] Ciencias Biológicas chemistry.chemical_compound ULTRASTRUCTURE Microscopy Electron Transmission Chlorophyta Toxicity Tests Botany PESTICIDE Bioassay Food science purl.org/becyt/ford/1.6 [https] EC50 biology Public Health Environmental and Occupational Health General Medicine Pesticide Bioquímica y Biología Molecular biology.organism_classification Pollution chemistry Thylakoid Chlorpyrifos Phytoplankton Toxicity Microscopy Electron Scanning Ultrastructure Biological Assay Green algae CIENCIAS NATURALES Y EXACTAS |
| Zdroj: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET Ecotoxicology and Environmental Safety, 2015, vol. 120, p. 334-341. Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) Universidad Nacional del Nordeste instacron:UNNE |
| Popis: | The effect of the organophosphorus insecticide chlorpyrifos on the growth, biovolume, and ultrastructure of the green microalga Ankistrodesmus gracilis was evaluated. Concentrations of 9.37, 18.75, 37.5, 75 and 150 mg L 1 of chlorpyrifos were assayed along with a control culture. At the end of the bioassay the ultrastructure of algal cells from control culture and from cultures exposed to 37.5 and 150 mg L 1 was observed under transmission (TEM) and scanning electron microscopy (SEM). After 24 and 48 h, treatments with 75 and 150 mg L 1 inhibited the growth of A. gracilis; whereas after 72 and 96 h, all the treatments except at 9.37 mg L 1 significantly affected the algae growth. The effective concentration 50 (EC50) after 96 h was 22.44 mg L 1 of chlorpyrifos. After the exposure to the insecticide, an increase in the biovolume was observed, with a larger increase in cells exposed to 75 and 150 mg L 1 . Radical changes were observed in the ultrastructure of cells exposed to chlorpyrifos. The insecticide affected the cell shape and the distribution of the crests in the wall. At 37.5 mg L 1 electodense bodies were observed along with an increase in the size and number of starch granules. At 150 mg L 1 such bodies occupied almost the whole cytoplasm together with lipids and remains of thylakoids. Autospores formation occurred normally at 37.5 mg L 1 while at 150 mg L 1 karyokinesis occurred, but cell-separation-phase was inhibited. The present study demonstrates that the exposure of phytoplankton to the insecticide chlorpyrifos leads to effects observed at both cellular and population level. Fil: Asselborn, Viviana Magdalena. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Laboratorio de Ecología Acuática; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Fernandez, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto Argentino de Oceanografía. Universidad Nacional del Sur. Instituto Argentino de Oceanografía; Argentina Fil: Zalocar, Yolanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Centro de Ecología Aplicada del Litoral. Universidad Nacional del Nordeste. Centro de Ecología Aplicada del Litoral; Argentina Fil: Parodi, Elisa Rosalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto Argentino de Oceanografía. Universidad Nacional del Sur. Instituto Argentino de Oceanografía; Argentina |
| Databáze: | OpenAIRE |
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