Synergistic antiviral effects of ribavirin and the C-nucleoside analogs tiazofurin and selenazofurin against togaviruses, bunyaviruses, and arenaviruses
| Autor: | P. G. Canonico, Roland K. Robins, John W. Huggins |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
Bunyaviridae
viruses Antiviral Agents Virus Selenium chemistry.chemical_compound IMP Dehydrogenase Organoselenium Compounds Ribavirin Togaviridae medicine Pharmacology (medical) Rift Valley fever Arenaviridae Pharmacology Virus quantification Arenavirus biology Yellow fever Drug Synergism Japanese encephalitis medicine.disease biology.organism_classification Virology Drug Combinations Infectious Diseases chemistry Ribonucleosides Tiazofurin Research Article medicine.drug |
| Zdroj: | Antimicrobial Agents and Chemotherapy. 26:476-480 |
| ISSN: | 1098-6596 0066-4804 |
| DOI: | 10.1128/aac.26.4.476 |
| Popis: | Binary combinations of the N-nucleoside ribavirin (1-beta-D-ribofuranosyl-1,2,4-triazole-3-carboxamide) and the C-nucleoside analog selenazofurin (2-beta-D-ribofuranosylselenazole-4-carboxamide) or tiazofurin (2-beta-D-ribofuranosylthiazole-4-carboxamide) were tested in vitro for activity against Venezuelan equine encephalomyelitis, Japanese encephalitis, yellow fever, Rift Valley fever, Korean hemorrhagic fever, and Pichinde viruses. The 50% effective dose for each compound alone or in a series of combinations was determined with a plaque reduction assay. Combinations of ribavirin and selenazofurin were synergistic against Venezuelan equine encephalomyelitis, Japanese encephalitis, yellow fever, and Pichinde viruses, with fractional inhibitory concentrations of 0.1, 0.2, 0.4, 0.4, respectively, but showed additive effects against Korean hemorrhagic fever and Rift Valley fever viruses. Combinations of ribavirin and tiazofurin were synergistic against yellow fever and Japanese encephalitis (fractional inhibitory concentrations, 0.41 and 0.48, respectively) but showed additive effects against Korean hemorrhagic fever virus. Combinations of selenazofurin and tiazofurin had additive effects against Japanese encephalitis, yellow fever, and Korean hemorrhagic fever viruses. The effect of combinations on cell toxicity was additive, both in monolayers of nondividing cells incubated under agar for the same period as the plaque assay and for rapidly dividing cells given short-term exposure (4 h), followed by determination of the proportion of surviving cells with a colony forming assay. |
| Databáze: | OpenAIRE |
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