Structural and functional analysis of transcriptional control of the Rhodobacter capsulatus puf operon
| Autor: | Camellia W. Adams, Stanley N. Cohen, J T Beatty, M E Forrest |
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| Rok vydání: | 1989 |
| Předmět: |
Transcription
Genetic Protein Conformation Operon Base pair Molecular Sequence Data Photosynthetic Reaction Center Complex Proteins Restriction Mapping Microbiology Protein structure Bacterial Proteins Transcription (biology) Amino Acid Sequence Promoter Regions Genetic Molecular Biology Gene Genetics Rhodobacter Base Sequence biology Nucleic acid sequence beta-Galactosidase biology.organism_classification Rhodopseudomonas Genes Genes Bacterial Photosynthetic membrane Research Article |
| Zdroj: | Journal of Bacteriology. 171:473-482 |
| ISSN: | 1098-5530 0021-9193 |
| DOI: | 10.1128/jb.171.1.473-482.1989 |
| Popis: | We report data indicating that the Rhodobacter capsulatus puf operon promoter and the site for its oxygen regulation are located more than 700 base pairs upstream from the previously identified puf genes and have identified the nucleotide sequences that constitute these control signals. A model is proposed in which a polycistronic transcript at least 3.4 kilobases in length is initiated near the O2-regulated promoter and is processed posttranscriptionally by endonucleolytic cleavage at multiple sites, yielding discrete mRNA segments that are degraded at different rates. A newly identified gene (pufQ), which includes a hydrophobic domain having some similarity to domains of the products of the pufL and pufM genes, begins 313 nucleotides into the puf transcript and is located entirely within the most rapidly degraded segment of the transcript. A previously identified puf transcript segment encoding structural proteins for photosynthetic membrane complexes persists after degradation of the most 5' region of the transcript and is itself subject to segmentally specific degradation. Our results suggest a model in which differential expression of the multiple genes encoded by the puf operon is at least in part attributable to major differences in the rates of decay of the various segments of puf mRNA. |
| Databáze: | OpenAIRE |
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