3D Printed Microfluidic Device with Integrated Biosensors for Online Analysis of Subcutaneous Human Microdialysate
| Autor: | Guang-Zhong Yang, Vincenzo F. Curto, Pankaj Vadgama, Martyn G. Boutelle, Carlo A. Seneci, Salzitsa Anastasova, Chu Wang, Sally A. N. Gowers |
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| Přispěvatelé: | Wellcome Trust, Engineering & Physical Science Research Council (EPSRC) |
| Rok vydání: | 2015 |
| Předmět: |
3d printed
Tissue concentrations Microdialysis Microfluidics Nanotechnology Biosensing Techniques 02 engineering and technology 01 natural sciences Article Analytical Chemistry Microfluidic Analysis Online analysis 0399 Other Chemical Sciences Humans Lactic Acid Electrodes RAPID SAMPLING MICRODIALYSIS ELECTROANALYSIS Monitoring Physiologic Science & Technology Chemistry Chemistry Analytical 010401 analytical chemistry Continuous monitoring VERSATILE Microfluidic Analytical Techniques 021001 nanoscience & nanotechnology NETWORKS 0104 chemical sciences Glucose Microfluidic chip Physical Sciences Printing Three-Dimensional 0210 nano-technology 0301 Analytical Chemistry Biosensor |
| Zdroj: | Analytical Chemistry |
| ISSN: | 1520-6882 0003-2700 |
| DOI: | 10.1021/acs.analchem.5b01353 |
| Popis: | This work presents the design, fabrication, and characterization of a robust 3D printed microfluidic analysis system that integrates with FDA-approved clinical microdialysis probes for continuous monitoring of human tissue metabolite levels. The microfluidic device incorporates removable needle type integrated biosensors for glucose and lactate, which are optimized for high tissue concentrations, housed in novel 3D printed electrode holders. A soft compressible 3D printed elastomer at the base of the holder ensures a good seal with the microfluidic chip. Optimization of the channel size significantly improves the response time of the sensor. As a proof-of-concept study, our microfluidic device was coupled to lab-built wireless potentiostats and used to monitor real-time subcutaneous glucose and lactate levels in cyclists undergoing a training regime. |
| Databáze: | OpenAIRE |
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