Analysis of the structure and expression of the chicken gene encoding a homolog of the human RREB-1 transcription factor
| Autor: | Jon H. Miyake, Daniel P. Szeto, William E. Stumph |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
DNA
Complementary Molecular Sequence Data Sequence alignment Biology DNA-binding protein Sequence Homology Nucleic Acid Genetics Animals Humans Amino Acid Sequence Gene Peptide sequence Transcription factor Cloning Zinc finger Base Sequence Sequence Homology Amino Acid Zinc Fingers General Medicine Molecular biology DNA-Binding Proteins Blotting Southern Organ Specificity ras Proteins Signal transduction Chickens Sequence Alignment Transcription Factors |
| Zdroj: | Gene. 202:177-186 |
| ISSN: | 0378-1119 |
| DOI: | 10.1016/s0378-1119(97)00491-5 |
| Popis: | Ras proteins are involved in a number of signal transduction pathways including the mitogen-activated kinase cascade. Activated MAPKs translocate to the nucleus and phosphorylate transcription factors such as c-myc, TCF and AP-1. Recently, a Ras-responsive element binding transcription factor, RREB-1, was cloned from a human medullary thyroid carcinoma cell line. RREB-1 is a zinc finger protein that binds to a Ras-responsive element in the promoter of the human calcitonin gene. We report the cloning of the chicken homologue to human RREB-1. Amino-acid alignment demonstrates that chicken and human RREB-1 are 53% identical and 69% similar. Genomic southern analysis indicates that chicken rreb-1 is a single-copy gene in the chicken genome. We demonstrate that chicken and human rreb-1 display the same tissue distribution, being expressed in all tissues examined except the brain. Interestingly, chicken RREB-1 has an extended N-terminus and contains 16 zinc fingers of the TFIIIA subclass, in comparison to human RREB-1 which was reported to contain only four zinc fingers. The size discrepancy between the two predicted gene products is further discussed. An unusual structural feature of RREB-1 is the widely spaced arrangement of the zinc fingers. |
| Databáze: | OpenAIRE |
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