The evaluation of hypoxia-inducible factor 1 in N-nitro-L-arginine methyl ester preeclampsia model of pregnant rats
| Autor: | Ali Kaya, Ahmet Sami Güven, Zekeriya Küçükdurmaz, Abdullah Boztosun, Ömer Cevit, Sefa Gulturk, Hulya Seckin |
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| Přispěvatelé: | [Kaya, Ali] Cumhuriyet Univ, Tip Fak, Cocuk Sagligi & Hastaliklan AD, Dept Pediat,Sch Med, TR-58140 Sivas, Turkey -- [Boztosun, Abdullah] Cumhuriyet Univ, Sch Med, Dept Obstet & Gynecol, TR-58140 Sivas, Turkey -- [Seckin, Hulya] Sivas State Hosp, Dept Pediat, Sivas, Turkey -- [Kucukdurmaz, Zekeriya] Cumhuriyet Univ, Sch Med, Dept Cardiol, TR-58140 Sivas, Turkey -- [Gulturk, Sefa] Cumhuriyet Univ, Sch Med, Dept Physiol, TR-58140 Sivas, Turkey |
| Rok vydání: | 2011 |
| Předmět: |
medicine.medical_specialty
Systole Diastole Stimulation Blood Pressure Urine hypoxia-inducible factor 1 General Biochemistry Genetics and Molecular Biology Preeclampsia preeclampsia Fetus Pre-Eclampsia Pregnancy Internal medicine Medicine Animals Humans rat Rats Wistar Proteinuria business.industry Body Weight General Medicine Hypoxia (medical) medicine.disease Rats Trophoblasts Disease Models Animal Endocrinology NG-Nitroarginine Methyl Ester Immunohistochemistry Gestation Female Hypoxia-Inducible Factor 1 medicine.symptom business |
| Zdroj: | Journal of investigative medicine : the official publication of the American Federation for Clinical Research. 59(8) |
| ISSN: | 1708-8267 |
| Popis: | WOS: 000297247000020 PubMed ID: 21941213 Objective: The objective of the study was to evaluate hypoxia-inducible factor 1 (HIF-1), which plays a major role in the stimulation of angiogenesis in placental tissues, by using immunohistochemical staining in preeclampsia model of rats, developed by N-nitro-L-arginine methyl ester (L-NAME) Methods: Thirty pregnant rats were randomized into 2 groups (n = 15 in each group) on day 10 of gestation. L-NAME was given to rats in the study group by gavage. On days 0, 10, and 20 of gestation, rats were weighted, and urine protein values and blood pressures were measured. Hypoxia-inducible factor 1 expressions were assessed with immunohistochemical staining by using avidin-biotin peroxidase via selecting preparation. Results: Systolic and diastolic blood pressures and urine protein value of L-NAME group on day 20 of gestation were found to be significantly higher than those obtained on days 0 and 10 of gestation in the same group and those obtained on day 20 of gestation in the sham group (P < 0.05). Maternal weight, number of fetuses, and mean fetal weight of rats in L-NAME group on day 20 of gestation were found to be significantly lower than those obtained from rats in the sham group (P < 0.05). Regarding HIF-1 expression of placental tissues, mild immunohistochemical staining was found in 2 rats (13.4%) and moderate in 13 rats (86.6%) in the L-NAME group. A significant difference was found in terms of HIF-1 positivity in the maternal placentas of both groups (P < 0.05). Conclusions: L-NAME preeclampsia model of pregnant rats is consistent with human preeclampsia in terms of hypertension, proteinuria, and intrauterine growth retardation; in addition, it also shows evidence of placental hypoxia findings. |
| Databáze: | OpenAIRE |
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