Autoregulation of actin synthesis in hepatocytes by transcriptional and posttranscriptional mechanisms
| Autor: | Rainer Maria Bohle, Petra Dunker, Norbert Katz, Manfred Kröger, Ingo Just, Karl H. Reuner, Matthias Wiederhold |
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| Rok vydání: | 1995 |
| Předmět: |
Botulinum Toxins
Transcription Genetic Phalloidin Phalloidine macromolecular substances Biology Biochemistry Filamentous actin chemistry.chemical_compound Transcription (biology) medicine Animals Homeostasis RNA Messenger Rats Wistar Actin Cells Cultured Molecular biology Actins Cell biology Rats Cytosol medicine.anatomical_structure Profilin chemistry Liver Hepatocyte biology.protein Nucleus |
| Zdroj: | European journal of biochemistry. 230(1) |
| ISSN: | 0014-2956 |
| Popis: | Treatment of rat hepatocytes with the filamentous-actin-stabilizing toxin phalloidin decreased the amount of globular actin by 77% in the cytosol and by 80% in the nucleus within 12 h. Simultaneously, actin mRNA was specifically increased by 230%. The de-novo synthesis of actin mRNA, as measured by nuclear run-on transcription, was enhanced by 250%. Treatment of cells with actinomycin D blocked the increase of actin mRNA. The apparent half-life of actin mRNA was not significantly altered during treatment with phalloidin. In contrast, the globular-actin-stabilizing botulinum C2 toxin increased the amount of cytosolic globular actin by 50% within 12 h. Simultaneously, the actin mRNA level was decreased by 62%. However, de-novo synthesis of actin mRNA was not impaired. The apparent half-life of actin mRNA was decreased by approximately 60 % during treatment with C2 toxin. The data strongly suggest an autoregulatory control of actin synthesis on the basis of the globular/ filamentous actin ratio in rat hepatocytes at the transcriptional as well as at the posttranscriptional levels. |
| Databáze: | OpenAIRE |
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