An organoid biobank for childhood kidney cancers that captures disease and tissue heterogeneity
| Autor: | Calandrini, Camilla, Schutgens, Frans, Oka, Rurika, Margaritis, Thanasis, Candelli, Tito, Mathijsen, Luka, Ammerlaan, Carola, van Ineveld, Ravian L, Derakhshan, Sepide, de Haan, Sanne, Dolman, Emmy, Lijnzaad, Philip, Custers, Lars, Begthel, Harry, Kerstens, Hindrik H D, Visser, Lindy L, Rookmaaker, Maarten, Verhaar, Marianne, Tytgat, Godelieve A M, Kemmeren, Patrick, de Krijger, Ronald R, Al-Saadi, Reem, Pritchard-Jones, Kathy, Kool, Marcel, Rios, Anne C, van den Heuvel-Eibrink, Marry M, Molenaar, Jan J, van Boxtel, Ruben, Holstege, Frank C P, Clevers, Hans, Drost, Jarno, UU BETA RESEARCH, Faculteit Diergeneeskunde |
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| Přispěvatelé: | Paediatric Oncology, CCA - Cancer Treatment and Quality of Life, Hubrecht Institute for Developmental Biology and Stem Cell Research, UU BETA RESEARCH, Faculteit Diergeneeskunde |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male Pathology Renal Cell/drug therapy Genotyping Techniques Cell Culture Techniques General Physics and Astronomy Cell Culture Techniques/methods Nephroma Mesoblastic/drug therapy Stem cells Kidney Drug Screening Assays 0302 clinical medicine Kidney/pathology Tumor Cells Cultured RNA-Seq Precision Medicine lcsh:Science Child Rhabdoid Tumor/drug therapy Cancer Biological Specimen Banks Netherlands Adult stem cells Cultured Multidisciplinary Precision Medicine/methods Biobank Kidney Neoplasms Tumor Cells 3. Good health Organoids Gene Expression Regulation Neoplastic Mesoblastic/drug therapy Kidney Neoplasms/drug therapy 030220 oncology & carcinogenesis Child Preschool Female Single-Cell Analysis medicine.medical_specialty Stromal cell Adolescent Science Antitumor/methods Drug Screening Assays Antitumor/methods Transfection Wilms Tumor Article General Biochemistry Genetics and Molecular Biology Paediatric cancer 03 medical and health sciences Genetic Heterogeneity Young Adult medicine Organoid Carcinoma Humans Nephroma Kidney tumour Nephroma Mesoblastic Cancer models Preschool Carcinoma Renal Cell Rhabdoid Tumor Neoplastic Whole Genome Sequencing Genetic heterogeneity business.industry Infant General Chemistry DNA Methylation medicine.disease Organoids/pathology 030104 developmental biology Gene Expression Regulation Carcinoma Renal Cell/drug therapy Wilms Tumor/drug therapy lcsh:Q Drug Screening Assays Antitumor business Kidney cancer |
| Zdroj: | Nature Communications, 11(1). Nature Publishing Group Nature Communications, Vol 11, Iss 1, Pp 1-14 (2020) Nature communications, 11(1):1310. Nature Publishing Group Nature Communications |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/s41467-020-15155-6 |
| Popis: | Kidney tumours are among the most common solid tumours in children, comprising distinct subtypes differing in many aspects, including cell-of-origin, genetics, and pathology. Pre-clinical cell models capturing the disease heterogeneity are currently lacking. Here, we describe the first paediatric cancer organoid biobank. It contains tumour and matching normal kidney organoids from over 50 children with different subtypes of kidney cancer, including Wilms tumours, malignant rhabdoid tumours, renal cell carcinomas, and congenital mesoblastic nephromas. Paediatric kidney tumour organoids retain key properties of native tumours, useful for revealing patient-specific drug sensitivities. Using single cell RNA-sequencing and high resolution 3D imaging, we further demonstrate that organoid cultures derived from Wilms tumours consist of multiple different cell types, including epithelial, stromal and blastemal-like cells. Our organoid biobank captures the heterogeneity of paediatric kidney tumours, providing a representative collection of well-characterised models for basic cancer research, drug-screening and personalised medicine. Pre-clinical cell culture models capturing the heterogeneity of childhood kidney tumours are limited. Here, the authors establish and characterise an organoid biobank of tumour and matched normal organoid cultures from over 50 children with different subtypes of kidney cancer. |
| Databáze: | OpenAIRE |
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