Biphasic Effect of Transforming Growth Factor-β1 on in Vitro Angiogenesis
| Autor: | Lelio Orci, Roberto Montesano, Jean-Dominique Vassalli, Michael S. Pepper |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Cells
Cultured/drug effects Vascular Endothelial Growth Factor A medicine.medical_specialty Endothelium Angiogenesis medicine.medical_treatment Basic fibroblast growth factor Endothelium Vascular/cytology/ drug effects Endothelial Growth Factors Biology chemistry.chemical_compound Transforming Growth Factor beta Internal medicine medicine Animals ddc:576.5 Cells Cultured Lymphokines Dose-Response Relationship Drug Neovascularization Pathologic Vascular Endothelial Growth Factors Drug Synergism Cell Biology Endothelial stem cell Vascular endothelial growth factor Vascular endothelial growth factor A Endocrinology medicine.anatomical_structure Cytokine Transforming Growth Factor beta/ pharmacology chemistry Adrenal Cortex Cattle Fibroblast Growth Factor 2 Endothelium Vascular Adrenal Cortex/blood supply Transforming growth factor |
| Zdroj: | Experimental Cell Research, Vol. 204, No 2 (1993) pp. 356-363 |
| ISSN: | 0014-4827 |
| DOI: | 10.1006/excr.1993.1043 |
| Popis: | Although the existence of an increasing number of angiogenesis-regulating cytokines is well documented, the response elicited by combinations of these cytokines is largely unknown. Using an in vitro model in which microvascular endothelial cells can be induced to form capillary-like tubes within three-dimensional collagen or fibrin gels, we have investigated the effect of transforming growth factor-beta 1 (TGF-beta 1) on basic fibroblast growth factor (bFGF)-induced and vascular endothelial growth factor (VEGF)-induced angiogenesis. Endothelial cell invasion and capillary lumen formation were inhibited by TGF-beta 1 at relatively high concentrations (5-10 ng/ml), while lower concentrations (100 pg/ml-1 ng/ml) of TGF-beta 1 potentiated the effect of bFGF- and VEGF-induced invasion. The optimal potentiating effect was observed at 200-500 pg/ml TGF-beta 1. At invasion-potentiating doses of TGF-beta 1, lumen size in fibrin gels was markedly reduced compared to that in cultures treated with bFGF alone. These results show that TGF-beta 1 exerts a biphasic effect on bFGF- and VEGF-induced angiogenesis in vitro. Our studies support the notion that the nature of the angiogenic response elicited by a specific cytokine is contextual, i.e., depends on the presence and concentration of other cytokines in the pericellular environment of the responding endothelial cell. |
| Databáze: | OpenAIRE |
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