Propofol Administration During Early Postnatal Life Suppresses Hippocampal Neurogenesis
| Autor: | Tiande Yang, Zhiyong Du, Xiaohang Bao, Jing Huang, Xi Chen, Xiaotang Fan, Hong Li, Sheng Jing |
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| Rok vydání: | 2014 |
| Předmět: |
Male
medicine.medical_specialty Dendritic Spines Neurogenesis Neuroscience (miscellaneous) Hippocampus Apoptosis Biology Hippocampal formation Cell Line 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine Neural Stem Cells 030202 anesthesiology Internal medicine medicine Animals Protein kinase B Propofol Cell Proliferation Dentate gyrus SOXB1 Transcription Factors Cell Cycle Neurotoxicity medicine.disease Neural stem cell Enzyme Activation Mice Inbred C57BL Endocrinology Neurology Animals Newborn Bromodeoxyuridine Anesthesia Dentate Gyrus Female Mitogen-Activated Protein Kinases Proto-Oncogene Proteins c-akt 030217 neurology & neurosurgery medicine.drug Signal Transduction |
| Zdroj: | Molecular neurobiology. 53(2) |
| ISSN: | 1559-1182 |
| Popis: | Propofol is currently one of the most widely used intravenous anesthetics and has been indicated to induce cognitive dysfunction in adults. Here, we investigated the effects of propofol exposure during early postnatal life on hippocampal neurogenesis. Propofol (30 or 60 mg/kg) was administered to mice on either postnatal day (P) 7 or P7-P9; cell proliferation and neurogenesis in the dentate gyrus (DG) were evaluated on P8 or P17. It showed that exposure to propofol on P7 decreased hippocampal cell proliferation as indicated by BrdU and Sox2 immunostaining at P8 in propofol treatment at the dosage of 60 mg/kg but not at the dosage of 30 mg/kg. Western blots revealed propofol treatment decreased Akt or extracellular signal-related kinase (ERK) 1/2 phosphorylation in the hippocampus at P8. Propofol treatment on P7 to P9 reduced the numbers of newly formed neurons in the DG at P17, which was accompanied by delay of granule neuron maturation and decreased the density of dendritic spines, particularly the mushroom-shaped mature spines. Furthermore, the in vitro findings indicated propofol suppressed cell proliferation and cell mitosis and activated apoptosis of C17.2 neural stem cell line in a dose-dependent manner. These findings suggest that propofol impairs cell proliferation and inhibits neurogenesis in the immature mouse brain and thus is possibly involved in the cognitive dysfunction induced by propofol anesthesia. |
| Databáze: | OpenAIRE |
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