New Diagnostic Real-Time PCR for Specific Detection of Parachlamydia acanthamoebae DNA in Clinical Samples

Autor:	Klara M. Posfay-Barbe, Alain Gervaix, Gilbert Greub, Nicola Casson
Rok vydání:	2008
Předmět:	DNA Bacterial Male Microbiology (medical) Pathology medicine.medical_specialty Polymerase Chain Reaction/methods Adolescent Chlamydiology and Rickettsiology Specific detection Acanthamoeba Bronchiolitis/diagnosis/microbiology Biology Polymerase Chain Reaction Sensitivity and Specificity law.invention chemistry.chemical_compound law Gram-Negative Bacterial Infections/diagnosis/microbiology Parachlamydia acanthamoebae medicine Animals Humans DNA Bacterial/analysis Polymerase chain reaction ddc:618 Chlamydiales Infant Reproducibility of Results Acanthamoeba/microbiology medicine.disease biology.organism_classification Virology Chlamydiales/genetics/isolation & purification Real-time polymerase chain reaction chemistry Bronchiolitis Child Preschool Parachlamydia Female Gram-Negative Bacterial Infections DNA
Zdroj:	Journal of clinical microbiology, Vol. 46, No 4 (2008) pp. 1491-3
ISSN:	1098-660X 0095-1137
DOI:	10.1128/jcm.02302-07
Popis:	Given the low sensitivity of amoebal coculture, we developed a specific real-time PCR for the detection of Parachlamydia . The analytical sensitivity was high, and the inter- and intrarun variabilities were low. When the PCR was applied to nasopharyngeal aspirates, it was positive for six patients with bronchiolitis. Future studies should assess the role of Parachlamydia in bronchiolitis.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9085c1744d61c05c8f30d95e54f30e93 https://doi.org/10.1128/jcm.02302-07 Zobrazit plný text záznamu