Leishmanicidal Evaluation of Tetrahydroprotoberberine and Spirocyclic Erythrina-Alkaloids
| Autor: | Norberto Peporine Lopes, Luís Guilherme Pereira Feitosa, João Luis Callegari Lopes, Daniel R. Callejon, Thalita B. Riul, Achyut Adhikari, Lucas Miranda Marques, Denise Brentan Silva, Marcelo Dias Baruffi, Ram Lal Shrestha, Thais Guaratini |
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| Rok vydání: | 2014 |
| Předmět: |
Stereochemistry
ESPECTROSCOPIA DE MASSA Berberine Alkaloids Pharmaceutical Science Erythrina verna Article Cell Line Analytical Chemistry lcsh:QD241-441 Alkaloids lcsh:Organic chemistry Parasitic Sensitivity Tests Amphotericin B Drug Discovery medicine Humans Physical and Theoretical Chemistry Cytotoxicity Leishmaniasis IC50 Erythrina Leishmania Leishmania amazonensis biology Traditional medicine Plant Extracts Macrophages Organic Chemistry Corydalis govaniana spirocyclic erythrina-alkaloids Corydalis tetrahydro-protoberberine-type alkaloids medicine.disease biology.organism_classification In vitro Chemistry (miscellaneous) Molecular Medicine medicine.drug |
| Zdroj: | Molecules; Volume 19; Issue 5; Pages: 5692-5703 Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Molecules Molecules, Vol 19, Iss 5, Pp 5692-5703 (2014) |
| ISSN: | 1420-3049 |
| Popis: | Leishmaniasis is one of the World’s most problematic diseases in developing countries. Traditional medicines to treat leishmaniasis have serious side effects, as well as significant parasite resistance problems. In this work, two alkaloids 1 and 2 were obtained from Corydalis govaniana Wall and seven alkaloids 3–9, were obtained from Erythrina verna. The structures of the compounds were confirmed by mass spectrometry and 1D- and 2D-NMR spectroscopy. The leishmanicidal activity of compounds 1–9 against Leishmania amazonensis was tested on promastigote forms and cytotoxicity against J774 (macrophage cell line) was assessed in vitro. Compound 1 showed potent activity (IC50 = 0.18 µg/mL), compared with the standard amphotericin B (IC50 = 0.20 µg/mL). The spirocyclic erythrina-alkaloids showed lower leishmanicidal activity than dibenzoquinolizine type alkaloids. |
| Databáze: | OpenAIRE |
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