Rubia Gallega x Nelore crossbred cattle improve beef tenderness through changes in protein abundance and gene expression
Autor: | T. S. Martins, Cibele M. Prado, Tamyres Rodrigues de Amorim, Lenise Freitas Mueller, Juliano Coelho da Silveira, Pollyana Garbossa, Angélica Simone Cravo Pereira, Jéssica Luana Gemelli, Gabriella Vespe de Moura, Mirele Daiana Poleti, Fernando Baldi, Ingrid Harumi de Souza Fuzikawa, Adrielle Matias Ferrinho, Julián E. Muñoz |
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Přispěvatelé: | Universidade de São Paulo (USP), Universidade Estadual Paulista (UNESP) |
Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: | |
Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
Popis: | Made available in DSpace on 2022-04-28T19:42:38Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-09-01 The objective was to evaluate meat quality of Rubia Gallega x Nelore (RGN) crossbred compared to Nelore (N) cattle, using mRNA analysis of calpain 1 and 2, calpastatin, myostatin, and TGFβ; and protein abundance profile post mortem based on aged beef of the different genetic groups. Sixteen N and 16 RGN were finished in feedlot by 120 days and harvested. RGN beef presented greater tenderness. Transcripts analysis showed increased levels of TGFβ and MSTN in RGN, indicating differences in muscle fiber formation. However, no difference was observed in mRNA for CAPN1, CAPN2 and CAST. Protein analysis demonstrated increased troponin-T degradation in RGN. Interaction for HSP 27 degradation was observed, indicating the degradation of this protein is faster and greater in RGN than N. No difference in myostatin protein abundance was found. In general, RGN cattle improves beef quality and these differences may be explained by TGFβ and MSTN gene expression, troponin-T degradation and HSP abundance. Faculty of Animal Science and Food Engineering – University of São Paulo USP Faculty of Veterinary Medicine and Animal Science – University of São Paulo USP Faculty of Agricultural and Veterinary Sciences – São Paulo State University UNESP Faculty of Agricultural and Veterinary Sciences – São Paulo State University UNESP |
Databáze: | OpenAIRE |
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