Multi-site evaluation of the LN34 pan-lyssavirus real-time RT-PCR assay for post-mortem rabies diagnostics
| Autor: | Lisa Dettinger, Nina Gruhn, Tomy Joseph, Kimberly Wilkins, Victoria A. Olson, Pamela A. Yager, Mojgan Deldari, James W. Powell, Rolain Cadet, April D. Davis, Jinxin Gao, Kendra N. Pesko, Melanie Seiders, Kenneth Okogi, Mary G. Reynolds, Richard Griesser, William C. Carson, Mike Breckenridge, Crystal M. Gigante, Edson Michael M. Simon, Patricia Rabideau, Lillian A. Orciari, Kristina Hsieh, Ashutosh Wadhwa, Scott Brunt, Shrikrishna Isloor, Ryan M. Wallace, Maria Yna Joyce V. Chu, Yu Li, Veronica Yung Peredo, Rene Edgar Condori, Sujith S. Nath, Jeremiah T. Saliki, Susan Sanchez, Maria Carlos, Claire Hartloge |
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| Rok vydání: | 2018 |
| Předmět: |
RNA viruses
0301 basic medicine Viral Diseases lcsh:Medicine Artificial Gene Amplification and Extension Pathology and Laboratory Medicine medicine.disease_cause Polymerase Chain Reaction Geographical locations law.invention 0302 clinical medicine law Zoonoses Diagnosis Medicine and Health Sciences lcsh:Science Polymerase chain reaction Multidisciplinary biology Reverse Transcriptase Polymerase Chain Reaction Infectious Diseases Real-time polymerase chain reaction Medical Microbiology Research Design Viral Pathogens Viruses RNA Viral RNA extraction Pathogens Research Article Neglected Tropical Diseases Rabies 030231 tropical medicine Real-Time Polymerase Chain Reaction Microbiology Rabies Virus 03 medical and health sciences Extraction techniques medicine Animals Humans Molecular Biology Techniques Microbial Pathogens Molecular Biology Direct fluorescent antibody Lyssavirus Biology and life sciences business.industry lcsh:R Rabies virus Organisms Pilot Studies Reverse Transcriptase-Polymerase Chain Reaction Gold standard (test) Tropical Diseases medicine.disease biology.organism_classification Virology United States Research and analysis methods 030104 developmental biology North America lcsh:Q People and places business |
| Zdroj: | PLoS ONE PLoS ONE, Vol 13, Iss 5, p e0197074 (2018) |
| ISSN: | 1932-6203 |
| Popis: | Rabies is a fatal zoonotic disease that requires fast, accurate diagnosis to prevent disease in an exposed individual. The current gold standard for post-mortem diagnosis of human and animal rabies is the direct fluorescent antibody (DFA) test. While the DFA test has proven sensitive and reliable, it requires high quality antibody conjugates, a skilled technician, a fluorescence microscope and diagnostic specimen of sufficient quality. The LN34 pan-lyssavirus real-time RT-PCR assay represents a strong candidate for rabies post-mortem diagnostics due to its ability to detect RNA across the diverse Lyssavirus genus, its high sensitivity, its potential for use with deteriorated tissues, and its simple, easy to implement design. Here, we present data from a multi-site evaluation of the LN34 assay in 14 laboratories. A total of 2,978 samples (1,049 DFA positive) from Africa, the Americas, Asia, Europe, and the Middle East were tested. The LN34 assay exhibited low variability in repeatability and reproducibility studies and was capable of detecting viral RNA in fresh, frozen, archived, deteriorated and formalin-fixed brain tissue. The LN34 assay displayed high diagnostic specificity (99.68%) and sensitivity (99.90%) when compared to the DFA test, and no DFA positive samples were negative by the LN34 assay. The LN34 assay produced definitive findings for 80 samples that were inconclusive or untestable by DFA; 29 were positive. Five samples were inconclusive by the LN34 assay, and only one sample was inconclusive by both tests. Furthermore, use of the LN34 assay led to the identification of one false negative and 11 false positive DFA results. Together, these results demonstrate the reliability and robustness of the LN34 assay and support a role for the LN34 assay in improving rabies diagnostics and surveillance. |
| Databáze: | OpenAIRE |
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